Cloned (Comment) | Organism |
---|---|
gene fad7, DNA and amino acid sequence determination and analysis using gene fad3 as template. The fad7 and fad8 gene products are functionally equivalent, although the two genes share only about 75% nucleotide identity | Arabidopsis thaliana |
gene fad8, DNA and amino acid sequence determination and analysis using gene fad3 as template, constitutive expression of the fad8 cDNA in transgenic plants of a fad7 mutant JBl01, via Agrobacterium turnefaciens C58 (pGV3101) containing plasmid pBI/fad8 or pBIl2l, results in genetic complementation of the mutation, indicating that the fad7 and fad8 gene products are functionally equivalent. Expression of the fad8 cDNA in transgenic plants often results in the co-suppression of both the endogenous fad7 and fad8 genes in spite of the fact that these two genes share only about 75% nucleotide identity | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | constitutive expression of the fad8 cDNA in transgenic plants of a fad7 mutant JBl01, via Agrobacterium turnefaciens C58 (pGV3101) containing plasmid pBI/fad8 or pBIl2l, results in genetic complementation of the mutation, indicating that the fad7 and fad8 gene products are functionally equivalent. Expression of the fad8 cDNA in transgenic plants often results in the co-suppression of both the endogenous fad7 and fad8 genes in spite of the fact that these two genes share only about 75% nucleotide identity | Arabidopsis thaliana |
W149stop | the fads-1 mutation creates a premature stop codon 149 amino acids from the N-terminal end of the fad8 open reading frame, the mutation results in a complete loss of fad8 activity | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast membrane | membrane-associated | Arabidopsis thaliana | 31969 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50134 | - |
x * 50134, sequence calculation | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | P46310 | gene fad7 | - |
Arabidopsis thaliana | P48622 | gene FAD8 | - |
Arabidopsis thaliana Col-0 | P46310 | gene fad7 | - |
Arabidopsis thaliana Col-0 | P48622 | gene FAD8 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
Subunits | Comment | Organism |
---|---|---|
? | x * 50134, sequence calculation | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
FAD7 | - |
Arabidopsis thaliana |
FAD8 | - |
Arabidopsis thaliana |
omega-3 desaturase | - |
Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | the steady-state level of fad8 mRNA is strongly increased in plants grown at low temperature | up |
General Information | Comment | Organism |
---|---|---|
physiological function | the role of fad8 is to provide increased omega3 desaturase activity in plants that are exposed to low growth temperature | Arabidopsis thaliana |