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Literature summary for 1.14.19.35 extracted from

  • Gibson, S.; Arondel, V.; Iba, K.; Somerville, C.
    Cloning of a temperature-regulated gene encoding a chloroplast omega-3 desaturase from Arabidopsis thaliana (1994), Plant Physiol., 106, 1615-1621.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene fad7, DNA and amino acid sequence determination and analysis using gene fad3 as template. The fad7 and fad8 gene products are functionally equivalent, although the two genes share only about 75% nucleotide identity Arabidopsis thaliana
gene fad8, DNA and amino acid sequence determination and analysis using gene fad3 as template, constitutive expression of the fad8 cDNA in transgenic plants of a fad7 mutant JBl01, via Agrobacterium turnefaciens C58 (pGV3101) containing plasmid pBI/fad8 or pBIl2l, results in genetic complementation of the mutation, indicating that the fad7 and fad8 gene products are functionally equivalent. Expression of the fad8 cDNA in transgenic plants often results in the co-suppression of both the endogenous fad7 and fad8 genes in spite of the fact that these two genes share only about 75% nucleotide identity Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information constitutive expression of the fad8 cDNA in transgenic plants of a fad7 mutant JBl01, via Agrobacterium turnefaciens C58 (pGV3101) containing plasmid pBI/fad8 or pBIl2l, results in genetic complementation of the mutation, indicating that the fad7 and fad8 gene products are functionally equivalent. Expression of the fad8 cDNA in transgenic plants often results in the co-suppression of both the endogenous fad7 and fad8 genes in spite of the fact that these two genes share only about 75% nucleotide identity Arabidopsis thaliana
W149stop the fads-1 mutation creates a premature stop codon 149 amino acids from the N-terminal end of the fad8 open reading frame, the mutation results in a complete loss of fad8 activity Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast membrane membrane-associated Arabidopsis thaliana 31969
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Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50134
-
x * 50134, sequence calculation Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P46310 gene fad7
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Arabidopsis thaliana P48622 gene FAD8
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Arabidopsis thaliana Col-0 P46310 gene fad7
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Arabidopsis thaliana Col-0 P48622 gene FAD8
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Source Tissue

Source Tissue Comment Organism Textmining
leaf
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Arabidopsis thaliana
-

Subunits

Subunits Comment Organism
? x * 50134, sequence calculation Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
FAD7
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Arabidopsis thaliana
FAD8
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Arabidopsis thaliana
omega-3 desaturase
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Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana the steady-state level of fad8 mRNA is strongly increased in plants grown at low temperature up

General Information

General Information Comment Organism
physiological function the role of fad8 is to provide increased omega3 desaturase activity in plants that are exposed to low growth temperature Arabidopsis thaliana