Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.19.35 extracted from

  • Teixeira, M.; Carvalho, I.; Brodelius, M.
    omega-3 Fatty acid desaturase genes isolated from purslane (Portulaca oleracea L.): Expression in different tissues and response to cold and wound stress (2010), J. Agric. Food Chem., 58, 1870-1877.
    View publication on PubMed
Search for more literature for 1.14.19.35

Cloned(Commentary)

Cloned (Comment) Organism
gene fad7, DNA and amino acid sequence determination and analysis Portulaca oleracea
gene fad8, DNA and amino acid sequence determination and analysis Portulaca oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast membrane
-
 Portulaca oleracea 31969
-
additional information ubiquitous expression, tissue-specific levels Portulaca oleracea
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ Portulaca oleracea
-
 an alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Portulaca oleracea A8DTI8 gene fad7
-
Portulaca oleracea C6ZGG8 gene FAD8
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Portulaca oleracea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
 Portulaca oleracea an alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?

Subunits

Subunits Comment Organism
More three histidine clusters characteristic of fatty acid desaturases, a putative chloroplast transit peptide in the N-terminal, and three putative transmembrane domains are identified in the enzyme sequence Portulaca oleracea

Synonyms

Synonyms Comment Organism
FAD7
-
 Portulaca oleracea
FAD8
-
 Portulaca oleracea
omega-3 fatty acid desaturase
-
 Portulaca oleracea

Cofactor

Cofactor Comment Organism Structure
Ferredoxin
-
 Portulaca oleracea

Expression

Organism Comment Expression
Portulaca oleracea FAD7 is up-regulated by wounding but not by low temperature. Total fatty acid and linolenic acid content are higher both, in wounded and intact leaves of plants exposed to low temperature up
Portulaca oleracea FAD8 is up-regulated by cold stress but not by wounding. Total fatty acid and linolenic acid content are higher both, in wounded and intact leaves of plants exposed to low temperature up

General Information

General Information Comment Organism
evolution three histidine clusters characteristic of fatty acid desaturases, a putative chloroplast transit peptide in the N-terminal, and three putative transmembrane domains are identified in the enzyme sequence Portulaca oleracea