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Literature summary for 1.14.19.31 extracted from

  • Tripodi, K.E.; Buttigliero, L.V.; Altabe, S.G.; Uttaro, A.D.
    Functional characterization of front-end desaturases from trypanosomatids depicts the first polyunsaturated fatty acid biosynthetic pathway from a parasitic protozoan (2006), FEBS J., 273, 271-280.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis, recombinant functional expression in Saccharomyces cerevisiae strain HH3 Leishmania major
DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis, recombinant functional expression in Saccharomyces cerevisiae strain HH3 Trypanosoma cruzi
DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis, recombinant functional expression in Saccharomyces cerevisiae strain HH3 Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
kinetoplast
-
Leishmania major 20023
-
kinetoplast
-
Trypanosoma cruzi 20023
-
kinetoplast
-
Trypanosoma brucei 20023
-

Organism

Organism UniProt Comment Textmining
Leishmania major Q4QFK0 gene LMJF_14_1340
-
Leishmania major Friedlin Q4QFK0 gene LMJF_14_1340
-
Trypanosoma brucei Q38AQ3 can be classified as DELTA4 desaturase due to its HX3HH motif; gene Tb10.6k15.3610
-
Trypanosoma brucei 427 Q38AQ3 can be classified as DELTA4 desaturase due to its HX3HH motif; gene Tb10.6k15.3610
-
Trypanosoma cruzi Q4DDJ1 can be classified as DELTA4 desaturase due to its HX3HH motif; gene Tc00.1047053510181.20
-
Trypanosoma cruzi CL Brener Q4DDJ1 can be classified as DELTA4 desaturase due to its HX3HH motif; gene Tc00.1047053510181.20
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
22:4 DELTA7,10,13,16-docosatetraenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+
-
Trypanosoma brucei 22:5 DELTA4,7,10,13,16-docosapentaenoic acid + 2 ferricytochrome b5 + 2 H2O
-
?
22:4 DELTA7,10,13,16-docosatetraenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+
-
Trypanosoma brucei 427 22:5 DELTA4,7,10,13,16-docosapentaenoic acid + 2 ferricytochrome b5 + 2 H2O
-
?
22:5 DELTA7,10,13,16,19-docosapentaenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+
-
Trypanosoma brucei 22:6 DELTA4,7,10,13,16,19-docosahexaenoic acid + 2 ferricytochrome b5 + 2 H2O
-
?
22:5 DELTA7,10,13,16,19-docosapentaenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+
-
Trypanosoma brucei 427 22:6 DELTA4,7,10,13,16,19-docosahexaenoic acid + 2 ferricytochrome b5 + 2 H2O
-
?
docosa-7,10,13,16,19-pentaenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+
-
Leishmania major docosa-4,7,10,13,16,19-hexaenoic acid + 2 ferricytochrome b5 + 2 H2O
-
?
docosa-7,10,13,16,19-pentaenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+
-
Trypanosoma cruzi docosa-4,7,10,13,16,19-hexaenoic acid + 2 ferricytochrome b5 + 2 H2O
-
?
docosa-7,10,13,16,19-pentaenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+
-
Trypanosoma cruzi CL Brener docosa-4,7,10,13,16,19-hexaenoic acid + 2 ferricytochrome b5 + 2 H2O
-
?
docosa-7,10,13,16,19-pentaenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+
-
Leishmania major Friedlin docosa-4,7,10,13,16,19-hexaenoic acid + 2 ferricytochrome b5 + 2 H2O
-
?
docosa-7,10,13,16-tetraenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+
-
Leishmania major docosa-4,7,10,13,16-pentaenoic acid + 2 ferricytochrome b5 + 2 H2O
-
?
docosa-7,10,13,16-tetraenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+
-
Trypanosoma cruzi docosa-4,7,10,13,16-pentaenoic acid + 2 ferricytochrome b5 + 2 H2O
-
?
docosa-7,10,13,16-tetraenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+
-
Trypanosoma cruzi CL Brener docosa-4,7,10,13,16-pentaenoic acid + 2 ferricytochrome b5 + 2 H2O
-
?
docosa-7,10,13,16-tetraenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+
-
Leishmania major Friedlin docosa-4,7,10,13,16-pentaenoic acid + 2 ferricytochrome b5 + 2 H2O
-
?

Synonyms

Synonyms Comment Organism
DELTA4 desaturase
-
Leishmania major
DELTA4 desaturase
-
Trypanosoma cruzi
DELTA4 desaturase
-
Trypanosoma brucei

Cofactor

Cofactor Comment Organism Structure
cytochrome b5
-
Leishmania major
cytochrome b5
-
Trypanosoma cruzi
cytochrome b5
-
Trypanosoma brucei

General Information

General Information Comment Organism
evolution the enzyme belongs to the front-end desaturases, phylogenetic analysis, overview. The HX3HH motif can be used to a priori characterize the enzyme as front-end DELTA4 desaturase Leishmania major
evolution the enzyme belongs to the front-end desaturases, phylogenetic analysis, overview. The HX3HH motif can be used to a priori characterize the enzyme as front-end DELTA4 desaturase Trypanosoma cruzi
evolution the enzyme belongs to the front-end desaturases, phylogenetic analysis, overview. The HX3HH motif can be used to a priori characterize the enzyme as front-end DELTA4 desaturase Trypanosoma brucei
metabolism the enzyme is involved in the pathway for very-long-chain polyunsaturated fatty acid biosynthesis existing in the protozoan. The enzymes involved in polyunsaturated fatty acid biosynthesis (i.e. DELTA6, DELTA8, DELTA5 and DELTA4 desaturases) are named front-end desaturases, because they introduce a double bond between a pre-existent olefinic bond and the carboxyl end of the fatty acid molecule. A complete pathway for very-long-chain polyunsaturated fatty acid biosynthesis is functional in Leishmania major using DELTA6, DELTA5 and DELTA4 desaturases Leishmania major
metabolism the enzymes involved in polyunsaturated fatty acid biosynthesis (i.e. DELTA6, DELTA8, DELTA5 and DELTA4 desaturases) are named front-end desaturases, because they introduce a double bond between a pre-existent olefinic bond and the carboxyl end of the fatty acid molecule. In trypanosomes, only DELTA4-desaturases are present from a complete pathway for very-long-chain polyunsaturated fatty acid biosynthesis Trypanosoma cruzi
metabolism the enzymes involved in polyunsaturated fatty acid biosynthesis (i.e. DELTA6, DELTA8, DELTA5 and DELTA4 desaturases) are named front-end desaturases, because they introduce a double bond between a pre-existent olefinic bond and the carboxyl end of the fatty acid molecule. In trypanosomes, only DELTA4-desaturases are present from a complete pathway for very-long-chain polyunsaturated fatty acid biosynthesis, the enzyme is also able to use n-3 and n-6 fatty acid isomers Trypanosoma brucei