Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis, recombinant functional expression in Saccharomyces cerevisiae strain HH3 | Leishmania major |
DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis, recombinant functional expression in Saccharomyces cerevisiae strain HH3 | Trypanosoma cruzi |
DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis, recombinant functional expression in Saccharomyces cerevisiae strain HH3 | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
kinetoplast | - |
Leishmania major | 20023 | - |
kinetoplast | - |
Trypanosoma cruzi | 20023 | - |
kinetoplast | - |
Trypanosoma brucei | 20023 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania major | Q4QFK0 | gene LMJF_14_1340 | - |
Leishmania major Friedlin | Q4QFK0 | gene LMJF_14_1340 | - |
Trypanosoma brucei | Q38AQ3 | can be classified as DELTA4 desaturase due to its HX3HH motif; gene Tb10.6k15.3610 | - |
Trypanosoma brucei 427 | Q38AQ3 | can be classified as DELTA4 desaturase due to its HX3HH motif; gene Tb10.6k15.3610 | - |
Trypanosoma cruzi | Q4DDJ1 | can be classified as DELTA4 desaturase due to its HX3HH motif; gene Tc00.1047053510181.20 | - |
Trypanosoma cruzi CL Brener | Q4DDJ1 | can be classified as DELTA4 desaturase due to its HX3HH motif; gene Tc00.1047053510181.20 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
22:4 DELTA7,10,13,16-docosatetraenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Trypanosoma brucei | 22:5 DELTA4,7,10,13,16-docosapentaenoic acid + 2 ferricytochrome b5 + 2 H2O | - |
? | |
22:4 DELTA7,10,13,16-docosatetraenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Trypanosoma brucei 427 | 22:5 DELTA4,7,10,13,16-docosapentaenoic acid + 2 ferricytochrome b5 + 2 H2O | - |
? | |
22:5 DELTA7,10,13,16,19-docosapentaenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Trypanosoma brucei | 22:6 DELTA4,7,10,13,16,19-docosahexaenoic acid + 2 ferricytochrome b5 + 2 H2O | - |
? | |
22:5 DELTA7,10,13,16,19-docosapentaenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Trypanosoma brucei 427 | 22:6 DELTA4,7,10,13,16,19-docosahexaenoic acid + 2 ferricytochrome b5 + 2 H2O | - |
? | |
docosa-7,10,13,16,19-pentaenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Leishmania major | docosa-4,7,10,13,16,19-hexaenoic acid + 2 ferricytochrome b5 + 2 H2O | - |
? | |
docosa-7,10,13,16,19-pentaenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Trypanosoma cruzi | docosa-4,7,10,13,16,19-hexaenoic acid + 2 ferricytochrome b5 + 2 H2O | - |
? | |
docosa-7,10,13,16,19-pentaenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Trypanosoma cruzi CL Brener | docosa-4,7,10,13,16,19-hexaenoic acid + 2 ferricytochrome b5 + 2 H2O | - |
? | |
docosa-7,10,13,16,19-pentaenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Leishmania major Friedlin | docosa-4,7,10,13,16,19-hexaenoic acid + 2 ferricytochrome b5 + 2 H2O | - |
? | |
docosa-7,10,13,16-tetraenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Leishmania major | docosa-4,7,10,13,16-pentaenoic acid + 2 ferricytochrome b5 + 2 H2O | - |
? | |
docosa-7,10,13,16-tetraenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Trypanosoma cruzi | docosa-4,7,10,13,16-pentaenoic acid + 2 ferricytochrome b5 + 2 H2O | - |
? | |
docosa-7,10,13,16-tetraenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Trypanosoma cruzi CL Brener | docosa-4,7,10,13,16-pentaenoic acid + 2 ferricytochrome b5 + 2 H2O | - |
? | |
docosa-7,10,13,16-tetraenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Leishmania major Friedlin | docosa-4,7,10,13,16-pentaenoic acid + 2 ferricytochrome b5 + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DELTA4 desaturase | - |
Leishmania major |
DELTA4 desaturase | - |
Trypanosoma cruzi |
DELTA4 desaturase | - |
Trypanosoma brucei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome b5 | - |
Leishmania major | |
cytochrome b5 | - |
Trypanosoma cruzi | |
cytochrome b5 | - |
Trypanosoma brucei |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the front-end desaturases, phylogenetic analysis, overview. The HX3HH motif can be used to a priori characterize the enzyme as front-end DELTA4 desaturase | Leishmania major |
evolution | the enzyme belongs to the front-end desaturases, phylogenetic analysis, overview. The HX3HH motif can be used to a priori characterize the enzyme as front-end DELTA4 desaturase | Trypanosoma cruzi |
evolution | the enzyme belongs to the front-end desaturases, phylogenetic analysis, overview. The HX3HH motif can be used to a priori characterize the enzyme as front-end DELTA4 desaturase | Trypanosoma brucei |
metabolism | the enzyme is involved in the pathway for very-long-chain polyunsaturated fatty acid biosynthesis existing in the protozoan. The enzymes involved in polyunsaturated fatty acid biosynthesis (i.e. DELTA6, DELTA8, DELTA5 and DELTA4 desaturases) are named front-end desaturases, because they introduce a double bond between a pre-existent olefinic bond and the carboxyl end of the fatty acid molecule. A complete pathway for very-long-chain polyunsaturated fatty acid biosynthesis is functional in Leishmania major using DELTA6, DELTA5 and DELTA4 desaturases | Leishmania major |
metabolism | the enzymes involved in polyunsaturated fatty acid biosynthesis (i.e. DELTA6, DELTA8, DELTA5 and DELTA4 desaturases) are named front-end desaturases, because they introduce a double bond between a pre-existent olefinic bond and the carboxyl end of the fatty acid molecule. In trypanosomes, only DELTA4-desaturases are present from a complete pathway for very-long-chain polyunsaturated fatty acid biosynthesis | Trypanosoma cruzi |
metabolism | the enzymes involved in polyunsaturated fatty acid biosynthesis (i.e. DELTA6, DELTA8, DELTA5 and DELTA4 desaturases) are named front-end desaturases, because they introduce a double bond between a pre-existent olefinic bond and the carboxyl end of the fatty acid molecule. In trypanosomes, only DELTA4-desaturases are present from a complete pathway for very-long-chain polyunsaturated fatty acid biosynthesis, the enzyme is also able to use n-3 and n-6 fatty acid isomers | Trypanosoma brucei |