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Literature summary for 1.14.19.29 extracted from

  • Garcia-Maroto, F.; Garrido-Cardenas, J.A.; Michaelson, L.V.; Napier, J.A.; Alonso, D.L.
    Cloning and molecular characterisation of a DELTA8-sphingolipid-desaturase from Nicotiana tabacum closely related to DELTA6-acyl-desaturases (2007), Plant Mol. Biol., 64, 241-250.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain W303-A1 Nicotiana tabacum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+ Nicotiana tabacum
-
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+ Nicotiana tabacum
-
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
?

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum A4GK73 variety Wisconsin-38
-

Source Tissue

Source Tissue Comment Organism Textmining
flower actively growing flower Nicotiana tabacum
-
fruit
-
Nicotiana tabacum
-
leaf young leaf Nicotiana tabacum
-
additional information no detectable expression is observed in roots, mature leaves, small flower buds nor in fully developed flowers at anthesis Nicotiana tabacum
-
seed
-
Nicotiana tabacum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
-
Nicotiana tabacum (4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
-
Nicotiana tabacum (4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
?
additional information the enzyme lacks DELTA6-desaturase activity Nicotiana tabacum ?
-
?

Synonyms

Synonyms Comment Organism
DELTA8-sphingolipid-desaturase
-
Nicotiana tabacum
DXDES
-
Nicotiana tabacum

Cofactor

Cofactor Comment Organism Structure
cytochrome b5
-
Nicotiana tabacum