Literature summary for 1.14.19.29 extracted from

Sperling, P.; Libisch, B.; Zahringer, U.; Napier, J.A.; Heinz, E.
Functional identification of a DELTA8-sphingolipid desaturase from Borago officinalis (2001), Arch. Biochem. Biophys., 388, 293-298.

Search for more literature for 1.14.19.29

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Saccharomyces cerevisiae strain w303-1a	Borago officinalis
expression of this openreading frame in Saccharomyces cerevisiae results in the formation of DELTA8-trans/cis-phytosphingenines not present in wild-type cells	Borago officinalis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
C18-phytosphinganine + ferrocytochrome b5 + O2 + H+	Borago officinalis	-	C18-8-phytosphingenine + ferricytochrome b5 + H2O	-	?
sphinganine + ferrocytochrome b5 + O2 + H+	Borago officinalis	-	8-sphingenine + ferricytochrome b5 + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Borago officinalis	-	-	-
Borago officinalis	Q9FR82	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+ = a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O	(1)	Borago officinalis	a
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+ = a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O	(2)	Borago officinalis	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	primarily expressed in young leaves	Borago officinalis	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
C18-phytosphinganine + ferrocytochrome b5 + O2 + H+	-	Borago officinalis	C18-8-phytosphingenine + ferricytochrome b5 + H2O	-	?
sphinganine + ferrocytochrome b5 + O2 + H+	-	Borago officinalis	8-sphingenine + ferricytochrome b5 + H2O	-	?

Synonyms

Synonyms	Comment	Organism
8 fatty acid desaturase	-	Borago officinalis
8-sphingolipid desaturase	-	Borago officinalis
DELTA8-sphingolipid desaturase	-	Borago officinalis
DES8	-	Borago officinalis

Cofactor

Cofactor	Comment	Organism	Structure
cytochrome b5	-	Borago officinalis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)