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Literature summary for 1.14.19.20 extracted from

  • Taton, M.; Husselstein, T.; Benveniste, P.; Rahier, A.
    Role of highly conserved residues in the reaction catalyzed by recombinant DELTA7-sterol-C5(6)-desaturase studied by site-directed mutagenesis (2000), Biochemistry, 39, 701-711.
    View publication on PubMed
Search for more literature for 1.14.19.20

Cloned(Commentary)

Cloned (Comment) Organism
-
 Arabidopsis thaliana
expression in null mutant erg3 of Saccharomyces cerevisiae Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
G234A no activity Arabidopsis thaliana
G234A inactive mutant enzyme Arabidopsis thaliana
G234D 2% of wild-type activity Arabidopsis thaliana
G234D low activity Arabidopsis thaliana
H147E no activity Arabidopsis thaliana
H147E inactive mutant enzyme Arabidopsis thaliana
H147L no activity Arabidopsis thaliana
H147L inactive mutant enzyme Arabidopsis thaliana
H151E no activity Arabidopsis thaliana
H151E inactive mutant enzyme Arabidopsis thaliana
H151l no activity Arabidopsis thaliana
H151l inactive mutant enzyme Arabidopsis thaliana
H161E no activity Arabidopsis thaliana
H161E inactive mutant enzyme Arabidopsis thaliana
H161L no activity Arabidopsis thaliana
H161L inactive mutant enzyme Arabidopsis thaliana
H164L no activity Arabidopsis thaliana
H164L inactive mutant enzyme Arabidopsis thaliana
H165L no activity Arabidopsis thaliana
H165L inactive mutant enzyme Arabidopsis thaliana
H203L reduced activity Arabidopsis thaliana
H203L 13% of wild-type activity Arabidopsis thaliana
H222E reduced activity Arabidopsis thaliana
H222E 6% of wild-type activity Arabidopsis thaliana
H222L no activity Arabidopsis thaliana
H222L inactive mutant enzyme Arabidopsis thaliana
H238E no activity Arabidopsis thaliana
H238E inactive mutant enzyme Arabidopsis thaliana
H238L no activity Arabidopsis thaliana
H238L inactive mutant enzyme Arabidopsis thaliana
H241L no activity Arabidopsis thaliana
H241L inactive mutant enzyme Arabidopsis thaliana
H242L no activity Arabidopsis thaliana
H242L inactive mutant enzyme Arabidopsis thaliana
K115L 2times higher activity than wild-type Arabidopsis thaliana
K115L Km-value for 5alpha-cholest-7-en-3beta-ol is 2.8fold higher than that of wild-type activity Arabidopsis thaliana
P175A wild-type activity Arabidopsis thaliana
P175A as active as wild-type enzyme Arabidopsis thaliana
P175V 4times higher activity than wild-type Arabidopsis thaliana
P175V Km-value for 5alpha-cholest-7-en-3beta-ol is 2.7fold higher than that of wild-type activity Arabidopsis thaliana
P201A 25% of wild-type activity Arabidopsis thaliana
P201A 20% of wild-type activity Arabidopsis thaliana
T114I wild-type activity Arabidopsis thaliana
T114I | Km-value for 5alpha-cholest-7-en-3beta-ol is 7.5fold higher than that of wild-type activity Arabidopsis thaliana
T114S 28times higher activity than wild-type Arabidopsis thaliana
T114S Km-value for 5alpha-cholest-7-en-3beta-ol is 7.6fold higher than that of wild-type activity Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
CN- 0.003 mM, 50% inhibition of recombinant enzyme Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
 5alpha-Cholest-7-en-3beta-ol
-
 Arabidopsis thaliana
0.032
-
 5alpha-Cholest-7-en-3beta-ol 30°C, wild-type enzyme Arabidopsis thaliana
0.07
-
 5alpha-Cholest-7-en-3beta-ol P175a mutant enzyme Arabidopsis thaliana
0.07
-
 5alpha-Cholest-7-en-3beta-ol 30°C, mutant enzyme P175A Arabidopsis thaliana
0.086
-
 5alpha-Cholest-7-en-3beta-ol P175V mutant enzyme Arabidopsis thaliana
0.086
-
 5alpha-Cholest-7-en-3beta-ol 30°C, mutant enzyme P175V Arabidopsis thaliana
0.09
-
 5alpha-Cholest-7-en-3beta-ol K115L mutant enzyme Arabidopsis thaliana
0.09
-
 5alpha-Cholest-7-en-3beta-ol 30°C, mutant enzyme K115L Arabidopsis thaliana
0.24
-
 5alpha-Cholest-7-en-3beta-ol T114I mutant enzyme Arabidopsis thaliana
0.24
-
 5alpha-Cholest-7-en-3beta-ol 30°C, mutant enzyme T114I Arabidopsis thaliana
0.244
-
 5alpha-Cholest-7-en-3beta-ol T114S mutant enzyme Arabidopsis thaliana
0.244
-
 5alpha-Cholest-7-en-3beta-ol 30°C, mutant enzyme T114S Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q39208
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5alpha-cholest-7-en-3beta-ol + ferrocytochrmoe b5 + H+ + O2 NADH is more efficient than NADPH Arabidopsis thaliana cholesta-5,7-dien-3beta-ol + ferricytochrome b5 + H2O
-
 ?
5alpha-cholest-7-en-3beta-ol + ferrocytochrome b5 + H+ + O2 NADH is more efficient than NADPH Arabidopsis thaliana cholesta-5,7-dien-3beta-ol + ferricytochrome b5 + 2 H2O
-
 ?

Synonyms

Synonyms Comment Organism
5-DES
-
 Arabidopsis thaliana
DELTA7-sterol 5-desaturase
-
 Arabidopsis thaliana
DELTA7-sterol DELTA5-dehydrogenase
-
 Arabidopsis thaliana
DELTA7-sterol-C5(6)-desaturase
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NAD(P)H
-
 Arabidopsis thaliana
NADH NADH is more efficient than NADPH Arabidopsis thaliana
NADPH NADH is more efficient than NADPH Arabidopsis thaliana