Literature summary for 1.14.19.13 extracted from

Kotajima, T.; Shiraiwa, Y.; Suzuki, I.
Functional screening of a novel DELTA15 fatty acid desaturase from the coccolithophorid Emiliania huxleyi (2014), Biochim. Biophys. Acta, 1842, 1451-1458 .

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Cloned(Commentary)

Cloned (Comment)	Organism
gene EhDES15, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenic analysis, a codon-optimized EhDES15 gene lacking the 74 amino acid peptide serving as putative bipartite signal and transit peptides at the N-terminus is synthesized and expressed heterologously Synechocystis sp. PCC 6803 cells under control of the trc promoter leading to increased level of n-3 fatty acid species,which are produced at only low levels in wild-type cyanobacterial cells grown at 30°C, semi-quantitative PCR expression analysis. Expression of gene EhDES15 in a Synechocystis mutant lacking the desB gene causes accumulation of 18:3n-3 and 18:4n-3 in the transformed cells	Emiliania huxleyi

Cloned (Comment)

Organism

gene EhDES15, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenic analysis, a codon-optimized EhDES15 gene lacking the 74 amino acid peptide serving as putative bipartite signal and transit peptides at the N-terminus is synthesized and expressed heterologously Synechocystis sp. PCC 6803 cells under control of the trc promoter leading to increased level of n-3 fatty acid species,which are produced at only low levels in wild-type cyanobacterial cells grown at 30°C, semi-quantitative PCR expression analysis. Expression of gene EhDES15 in a Synechocystis mutant lacking the desB gene causes accumulation of 18:3n-3 and 18:4n-3 in the transformed cells

Emiliania huxleyi

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	-	Emiliania huxleyi	9507	-

Organism

Organism	UniProt	Comment	Textmining
Emiliania huxleyi	R1FY33	-	-
Emiliania huxleyi CCMP1516	R1FY33	-	-

Synonyms

Synonyms	Comment	Organism
DELTA15 desaturase	-	Emiliania huxleyi
DELTA15 fatty acid desaturase	-	Emiliania huxleyi
EhDES15	-	Emiliania huxleyi

General Information

General Information	Comment	Organism
metabolism	the enzyme is is thought to be involved in 18:5n-3 biosynthesis	Emiliania huxleyi
additional information	the enzyme contains a putative transit sequence for localization in chloroplasts and a DELTA6 desaturase-like domain, but it does not contain a cytochrome b5 domain nor typical His-boxes found in DELTA15 desaturases	Emiliania huxleyi