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Literature summary for 1.14.19.1 extracted from

  • Foot, M.; Jeffcoat, R.; Russell, N.
    Some properties, including the substrate in vivo, of the DELTA9-desaturase in Micrococcus cryophilus (1983), Biochem. J., 209, 345-353.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.19.1

Inhibitors

Inhibitors Comment Organism Structure
azide at high concentrations Psychrobacter urativorans
CN- at high concentrations Psychrobacter urativorans
glutathione reduced, not marked Psychrobacter urativorans
L-cysteine not marked Psychrobacter urativorans
o-phenanthroline partially inhibitory Psychrobacter urativorans

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound Psychrobacter urativorans 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 20-25% stimulation Psychrobacter urativorans
additional information no special ion requirement Psychrobacter urativorans
Zn2+ 20-25% stimulation Psychrobacter urativorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Psychrobacter urativorans biosynthesis of unsaturated fatty acids. Possible substrates: acyl-CoA, acyl-acyl-carrier and acyl chains of phospholipids. The substrate in vivo is saturated phospholipid ?
-
 ?

Organism

Organism UniProt Comment Textmining
Psychrobacter urativorans
-
 ATCC 15174
-

Reaction

Reaction Comment Organism Reaction ID
stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O incorporation of fatty acid into phospholipid. Enzyme may contain an essential thiol group. Endogenous saturated phospholipid is desaturated rapidly in all experiments in contrast with exogenous saturated phospholipid, which generally is not desaturated Psychrobacter urativorans
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information not acyl-CoA as substrate but phospholipid Psychrobacter urativorans ?
-
 ?
additional information biosynthesis of unsaturated fatty acids. Possible substrates: acyl-CoA, acyl-acyl-carrier and acyl chains of phospholipids. The substrate in vivo is saturated phospholipid Psychrobacter urativorans ?
-
 ?
stearate + AH2 + O2
-
 Psychrobacter urativorans oleate + A + 2 H2O
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
21
-
 assay at Psychrobacter urativorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.7
-
 but assay at pH 7.4, to standardize the experiments in vitro with those carried out with intact bacteria Psychrobacter urativorans

Cofactor

Cofactor Comment Organism Structure
additional information requires some other form of reduced cofactor than a reduced nicotinamide nucleotide, no special cofactor requirement Psychrobacter urativorans