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Literature summary for 1.14.18.3 extracted from
- Overexpression and purification of the particulate methane monooxygenase from Methylococcus capsulatus (Bath) (2011), Methods Enzymol., 495, 177-193.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Methylococcus capsulatus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | multi-copper enzyme with 14.1 copper atoms per protein, highest specific activity is observed wit 0.04 mM Cu2+ in the growth medium | Methylococcus capsulatus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE | Methylococcus capsulatus |
| 27000 | - |
1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE | Methylococcus capsulatus |
| 28328 | - |
1 * 42785 + 1 * 29073 + 1 * 28328, MALDI-TOF mass spectrometry | Methylococcus capsulatus |
| 28376 | - |
1 * 42786 + 1 * 29063 + 1 * 28376, calculated from amino acid sequence | Methylococcus capsulatus |
| 29063 | - |
1 * 42786 + 1 * 29063 + 1 * 28376, calculated from amino acid sequence | Methylococcus capsulatus |
| 29073 | - |
1 * 42785 + 1 * 29073 + 1 * 28328, MALDI-TOF mass spectrometry | Methylococcus capsulatus |
| 42785 | - |
1 * 42785 + 1 * 29073 + 1 * 28328, MALDI-TOF mass spectrometry | Methylococcus capsulatus |
| 42786 | - |
1 * 42786 + 1 * 29063 + 1 * 28376, calculated from amino acid sequence | Methylococcus capsulatus |
| 45000 | - |
1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE | Methylococcus capsulatus |
| 100000 | - |
apo-protein | Methylococcus capsulatus |
| 220000 | - |
purified pMMO-detergent complex, gel filtration | Methylococcus capsulatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylococcus capsulatus | - |
- |
- |
| Methylococcus capsulatus ATCC 33009 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Sepharose Fast Flow column chromatography, lysine agarose column chromatography, Sephacryl S-300HR gel filtration, QEA-Sephadex A-50 column chromatography, and Sephacryl S200 gel filtration, Superdex 200 gel filtration, or ammonium sulfate precipitation followed by Source 30Q column chroamtography | Methylococcus capsulatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| propylene + duroquinol + O2 | - |
Methylococcus capsulatus | propylene oxide + duroquinone + H2O | - |
? | |
| propylene + duroquinol + O2 | - |
Methylococcus capsulatus ATCC 33009 | propylene oxide + duroquinone + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterotrimer | 1 * 42785 + 1 * 29073 + 1 * 28328, MALDI-TOF mass spectrometry | Methylococcus capsulatus |
| heterotrimer | 1 * 42786 + 1 * 29063 + 1 * 28376, calculated from amino acid sequence | Methylococcus capsulatus |
| heterotrimer | 1 * 45000 + 1 * 27000 + 1 * 23000, SDS-PAGE | Methylococcus capsulatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| particulate methane monooxygenase | - |
Methylococcus capsulatus |
| pMMO | - |
Methylococcus capsulatus |
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Release 2023.1 (January 2023)
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