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Literature summary for 1.14.18.3 extracted from
- Copper-dioxygen complex mediated C-H bond oxygenation: relevance for particulate methane monooxygenase (pMMO) (2009), Curr. Opin. Chem. Biol., 13, 119-131.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Methylosinus trichosporium OB3b |
| heterotrimer of three subunits, pmoA, pmoB, and pmoC, with a solvent-exposed domain above the trans-membrane domain, which consists of alpha-helices | Methylococcus capsulatus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| copper | two-coordinate (His2) mononuclear copper site and an anomalous site modeled as a dinuclear copper cluster. The cluster has one Cu ion bound by two His imidazoles and another by an imidazole and amino group of the pmoB N-terminal His | Methylococcus capsulatus |  |
| Zinc | a zinc site, probably adventitious, as the crystallization medium requires the metal. Purified pMMO does not contain zinc in the trans-membrane domain | Methylococcus capsulatus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Methylococcus capsulatus | 16020 | - |
| membrane | - |
Methylosinus trichosporium OB3b | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| copper | an anomalous site modeled as a dinuclear copper cluster. The mononuclear copper site is absent (one His is not conserved), and the zinc replaced by a copper ion | Methylosinus trichosporium OB3b | |
| additional information | purified pMMO does not contain zinc in the trans-membrane domain | Methylosinus trichosporium OB3b |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylococcus capsulatus | - |
- |
- |
| Methylococcus capsulatus Bath | - |
- |
- |
| Methylosinus trichosporium OB3b | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methane + reduced acceptor + H* + O2 | - |
Methylococcus capsulatus | methanol + acceptor + H2O | - |
? | |
| methane + reduced acceptor + H* + O2 | - |
Methylosinus trichosporium OB3b | methanol + acceptor + H2O | - |
? | |
| methane + reduced acceptor + H* + O2 | - |
Methylococcus capsulatus Bath | methanol + acceptor + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterotrimer | crystallography | Methylococcus capsulatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| particulate methane monooxygenase | - |
Methylococcus capsulatus |
| particulate methane monooxygenase | - |
Methylosinus trichosporium OB3b |
| pMMO | - |
Methylococcus capsulatus |
| pMMO | - |
Methylosinus trichosporium OB3b |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Methylococcus capsulatus | |
| NADH | - |
Methylosinus trichosporium OB3b | |
| NADPH | - |
Methylococcus capsulatus | |
| NADPH | - |
Methylosinus trichosporium OB3b | |
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