Inhibitors | Comment | Organism | Structure |
---|---|---|---|
duroquinol | increasing duroquinol concentration above 70 mM causes almost total inhibition of enzyme activity | Methylococcus capsulatus | |
NaCl | decrease in activity might be due to reduced enzyme solubility with increasing NaCl concentrations | Methylococcus capsulatus | |
propylene oxide | product inhibition at higher concentration | Methylococcus capsulatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound | Methylococcus capsulatus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | the enzyme contains three Co2+ ions per enzyme molecule | Methylococcus capsulatus | |
Fe2+ | the enzyme contains one Fe2+ ion per enzyme molecule | Methylococcus capsulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
x * 47000 + x * 26000 + x * 23000, SDS-PAGE | Methylococcus capsulatus |
26000 | - |
x * 47000 + x * 26000 + x * 23000, SDS-PAGE | Methylococcus capsulatus |
47000 | - |
x * 47000 + x * 26000 + x * 23000, SDS-PAGE | Methylococcus capsulatus |
660000 | - |
solubilized enzyme, gel filtration | Methylococcus capsulatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methane + reduced acceptor + H* + O2 | Methylococcus capsulatus | - |
methanol + acceptor + H2O | - |
? | |
methane + reduced acceptor + H* + O2 | Methylococcus capsulatus M | - |
methanol + acceptor + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylococcus capsulatus | - |
- |
- |
Methylococcus capsulatus M | - |
- |
- |
Purification (Comment) | Organism |
---|---|
optimization of solubilization and purification procedure for the hydroxylase component of membrane-bound enzyme, purification to homogeneity involves solubilization by dodexylbeta-D-maltoside, ion exchange chromatography and gel filtration, the purification includes the loss of the reductase component | Methylococcus capsulatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
optimization of a coupled assay method with propylene as substrate and a duroquinol-based NADH regeneration system | Methylococcus capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methane + reduced acceptor + H* + O2 | - |
Methylococcus capsulatus | methanol + acceptor + H2O | - |
? | |
methane + reduced acceptor + H* + O2 | - |
Methylococcus capsulatus M | methanol + acceptor + H2O | - |
? | |
Mn2+ + H2O2 | - |
Methylococcus capsulatus | ? | - |
? | |
Mn2+ + H2O2 | - |
Methylococcus capsulatus M | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | x * 47000 + x * 26000 + x * 23000, SDS-PAGE | Methylococcus capsulatus |
Synonyms | Comment | Organism |
---|---|---|
MMO | - |
Methylococcus capsulatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 45 | - |
Methylococcus capsulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 7.3 | assay at | Methylococcus capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Methylococcus capsulatus |