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Literature summary for 1.14.18.3 extracted from
- Characterization and structural analysis of an active particulate methane monooxygenase trimer from Methylococcus capsulatus (Bath) (2005), Biochemistry, 44, 10954-10965.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | sMMO | Methylococcus capsulatus | 5737 | - |
| membrane | bound, pMMO | Methylococcus capsulatus | 16020 | - |
| additional information | two distinct enzyme forms exist: a soluble cytoplasmic sMMO and a membrane-bound particulate pMMO | Methylococcus capsulatus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| copper | required, activates | Methylococcus capsulatus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
1 * 47000 + 1 * 26000 + 1 * 23000, three-dimensional structure analysis of purified pMMO by electron microscopy and single-particle analysis at 23 A resolution, overview | Methylococcus capsulatus |
| 26000 | - |
1 * 47000 + 1 * 26000 + 1 * 23000, three-dimensional structure analysis of purified pMMO by electron microscopy and single-particle analysis at 23 A resolution, overview | Methylococcus capsulatus |
| 47000 | - |
1 * 47000 + 1 * 26000 + 1 * 23000, three-dimensional structure analysis of purified pMMO by electron microscopy and single-particle analysis at 23 A resolution, overview | Methylococcus capsulatus |
| 390000 | - |
gel filtration | Methylococcus capsulatus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methane + reduced acceptor + H* + O2 | Methylococcus capsulatus | - |
methanol + acceptor + H2O | - |
? | |
| methane + reduced acceptor + H* + O2 | Methylococcus capsulatus Bath | - |
methanol + acceptor + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylococcus capsulatus | - |
- |
- |
| Methylococcus capsulatus Bath | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| stable and active native pMMO from membranes, by solubilization with 1% w/v CHAPS, removal of soluble proteins, gel filtration, anion exchange chromatography, and ultrafiltration | Methylococcus capsulatus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.034 | - |
purified enzyme, substrates propylene and duroquinol | Methylococcus capsulatus |
| 0.114 | - |
membrane-bound enzyme, substrates propylene and duroquinol | Methylococcus capsulatus |
| 0.23 | - |
membrane-bound enzyme, substrates propylene and NADH | Methylococcus capsulatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methane + reduced acceptor + H* + O2 | - |
Methylococcus capsulatus | methanol + acceptor + H2O | - |
? | |
| methane + reduced acceptor + H* + O2 | - |
Methylococcus capsulatus Bath | methanol + acceptor + H2O | - |
? | |
| propene + reduced acceptor + H+ + O2 | - |
Methylococcus capsulatus | 1,2-epoxypropane + acceptor + H2O | - |
? | |
| propene + reduced acceptor + H+ + O2 | - |
Methylococcus capsulatus Bath | 1,2-epoxypropane + acceptor + H2O | - |
? | |
| propylene + duroquinol + O2 | - |
Methylococcus capsulatus | propylene epoxide + duroquinone + H2O | - |
? | |
| propylene + duroquinol + O2 | - |
Methylococcus capsulatus Bath | propylene epoxide + duroquinone + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 1 * 47000 + 1 * 26000 + 1 * 23000, three-dimensional structure analysis of purified pMMO by electron microscopy and single-particle analysis at 23 A resolution, overview | Methylococcus capsulatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| particulate methane monooxygenase | - |
Methylococcus capsulatus |
| pMMO | - |
Methylococcus capsulatus |
| pMMO hydroxylase | - |
Methylococcus capsulatus |
| pMMO-H | - |
Methylococcus capsulatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
assay at | Methylococcus capsulatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Methylococcus capsulatus |
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