Cloned (Comment) | Organism |
---|---|
genetic analysis of pMMO genes | Methylococcus capsulatus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | native occurence of a truncated form of pMMO with different molecular weight | Methylococcus capsulatus |
General Stability | Organism |
---|---|
pMMO is very unstable in vitro | Methylococcus capsulatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-bound particulate enzyme form termed pMMO | Methylococcus capsulatus | 16020 | - |
membrane | enzyme from cells grown under conditions of high copper availability is membrane-bound | Methylococcus capsulatus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu+ | pMMO, requirement for, contains 12-15 Cu+ ions per molecule of enzyme | Methylococcus capsulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
1 * 45000 + 1 * 26000 + 1 * 23000, pMMO, SDS-PAGE | Methylococcus capsulatus |
26000 | - |
1 * 45000 + 1 * 26000 + 1 * 23000, pMMO, SDS-PAGE | Methylococcus capsulatus |
45000 | - |
1 * 45000 + 1 * 26000 + 1 * 23000, pMMO, SDS-PAGE | Methylococcus capsulatus |
94000 | - |
pMMO | Methylococcus capsulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylococcus capsulatus | - |
Bath | - |
Methylococcus capsulatus | - |
enzyme form pMMO is copper-inducible | - |
Methylococcus capsulatus Bath | - |
Bath | - |
Oxidation Stability | Organism |
---|---|
sensitive to O2, 3 kinetically distinct froms of pMMO with respect to O2 tension, type I is stable with moderate activity, type II is highly unstable to oxygen, type III is an intermediate form | Methylococcus capsulatus |
Purification (Comment) | Organism |
---|---|
pMMO after induction with copper, kinetic type I with respect to O2-sensitivity | Methylococcus capsulatus |
Storage Stability | Organism |
---|---|
-80°C, pMMO kinetically type I with respect to O2-sensitivity, repeated freeze-thaw-cycles, stable | Methylococcus capsulatus |
Subunits | Comment | Organism |
---|---|---|
trimer | 1 * 45000 + 1 * 26000 + 1 * 23000, pMMO, SDS-PAGE | Methylococcus capsulatus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
or below: pMMO is irreversibly inactivated | Methylococcus capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Methylococcus capsulatus |