Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Priestia megaterium |
Protein Variants | Comment | Organism |
---|---|---|
R209H | the mutant exhibits a 1.7fold increase in monophenolase activity accompanied by a 1.5fold decrease in diphenolase activity, resulting in a 2.6fold improvement in the monophenolase/diphenolase activity ratio | Priestia megaterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
L-tyrosine | wild type enzyme, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C | Priestia megaterium | |
0.4 | - |
L-Dopa | wild type enzyme, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C | Priestia megaterium | |
0.62 | - |
L-Dopa | mutant enzyme R209H, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C | Priestia megaterium | |
1.2 | - |
D-Dopa | wild type enzyme, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C | Priestia megaterium | |
1.3 | - |
L-tyrosine | mutant enzyme R209H, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C | Priestia megaterium | |
3.8 | - |
D-Dopa | mutant enzyme R209H, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography, gel filtration | Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-DOPA + O2 | - |
Priestia megaterium | D-dopaquinone + H2O | - |
? | |
L-Dopa + O2 | - |
Priestia megaterium | L-dopaquinone + H2O | - |
? | |
L-tyrosine + O2 | - |
Priestia megaterium | L-DOPA + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
tyrosinase | - |
Priestia megaterium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.29 | - |
L-tyrosine | wild type enzyme, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C | Priestia megaterium | |
2.24 | - |
L-tyrosine | mutant enzyme R209H, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C | Priestia megaterium | |
6.35 | - |
D-Dopa | wild type enzyme, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C | Priestia megaterium | |
6.88 | - |
D-Dopa | mutant enzyme R209H, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C | Priestia megaterium | |
7.18 | - |
L-Dopa | mutant enzyme R209H, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C | Priestia megaterium | |
10.59 | - |
L-Dopa | wild type enzyme, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C | Priestia megaterium |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.72 | - |
L-tyrosine | mutant enzyme R209H, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C | Priestia megaterium | |
1.81 | - |
D-Dopa | mutant enzyme R209H, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C | Priestia megaterium | |
5.29 | - |
D-Dopa | wild type enzyme, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C | Priestia megaterium | |
11.58 | - |
L-Dopa | mutant enzyme R209H, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C | Priestia megaterium | |
26.47 | - |
L-Dopa | wild type enzyme, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C | Priestia megaterium | |
34.05 | - |
L-tyrosine | wild type enzyme, in 50 mM Tris-HCl buffer pH 7.0, 0.01 mM CuSO4, 28°C | Priestia megaterium |