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Literature summary for 1.14.18.1 extracted from

  • Chao, A.
    Preparation of porous chitosan/GPTMS hybrid membrane and its application in affinity sorption for tyrosinase purification with Agaricus bisporus (2008), J. Membr. Sci., 311, 306-318.
No PubMed abstract available

Application

Application Comment Organism
biotechnology prepared CSG1.0 possesses macropores which permit fluid to pass through, and micropores in the skeleton of macropores which increase the specific surface area for ligands to immobilize on. These hybrid membranes could be used to immobilize ligands for affinity sorption. Advanced application of the hybrid membranes can be developed for application in tissue engineering, enzyme immobilization, catalysts support, fuel cells, etc. Agaricus bisporus

Inhibitors

Inhibitors Comment Organism Structure
glutamic acid individually grafted onto a novel CSG1.0 membrane as a ligand for enzyme purification Agaricus bisporus
histidine individually grafted onto a novel CSG1.0 membrane as a ligand for enzyme purification Agaricus bisporus
p-Aminobenzoic acid individually grafted onto a novel CSG1.0 membrane as a ligand. This study indicates the p-aminobenzoic acid (ABA) grafted chitosan membrane (CSG-ABA) exhibits the best sorption capacity on tyrosinase Agaricus bisporus

Organism

Organism UniProt Comment Textmining
Agaricus bisporus
-
high enzyme content
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-3,4-dihydroxyphenylalanine + 1/2 O2 individually grafted onto a novel CSG1.0 membrane as a ligand Agaricus bisporus L-dopaquinone + H2O
-
?
L-tyrosine + O2 + AH2 individually grafted onto a novel CSG1.0 membrane as a ligand Agaricus bisporus L-3,4-dihydroxyphenylalanine + H2O + A
-
?

Synonyms

Synonyms Comment Organism
tyrosinase
-
Agaricus bisporus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
Agaricus bisporus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.052
-
p-Aminobenzoic acid
-
Agaricus bisporus
0.079
-
glutamic acid
-
Agaricus bisporus
0.148
-
histidine
-
Agaricus bisporus