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Literature summary for 1.14.18.1 extracted from

  • Han, H.Y.; Lee, J.R.; Xu, W.A.; Hahn, M.J.; Yang, J.M.; Park, Y.D.
    Effect of Cl- on tyrosinase: complex inhibition kinetics and biochemical implication (2007), J. Biomol. Struct. Dyn., 25, 165-171.
    View publication on PubMed
Search for more literature for 1.14.18.1

Cloned(Commentary)

Cloned (Comment) Organism
tyrosinase gene transfection, by calcium-phosphate precipitation method, into HEK293 cell significantly down-regulates the expression levels of solute carrier family 12, member 4 and 7, which are known to be Cl- transporters. Observation of significant pigmentation of the HEK293 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Cl- enzyme added to substrate L-dopa, that is mixed with various concentrations of NaCl in 0.05 M sodium phosphate buffer, pH 7.0. When the NaCl concentration reaches to 600 mM, almost all the enzyme activity is abolished. Cl- induced inhibition of human tyrosinase, Cl- inhibits tyrosinase in a slope-parabolic competitive manner. TXM13 originated tyrosinase and the overexpressed human tyrosinase in HEK293 cells are inhibited. The inhibition by Cl- is reversible Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.48
-
 L-Dopa human tyrosinase source HEK293-TYR Homo sapiens
0.49
-
 L-Dopa human tyrosinase source TXM13 pigment cell Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm The immunocytochemistry shows that the ectopic tyrosinase is located in the cytoplasm, it is propably the membrane-bounded form since no detection of enzyme activity with the soluble solution without Triton X-100 is possible, nor is it found in Western blotting Homo sapiens 5737
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
-
 Homo sapiens
-
TXM13 cell prepared from the TXM13 melanotic cells as well as from cells that had undergone gene transfection Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,6-dihydroxyindole + 1/2 O2
-
 Homo sapiens 1H-indole-5,6-dione + H2O
-
 ?
L-dopa + 1/2 O2
-
 Homo sapiens L-dopaquinone + H2O
-
 ?
L-tyrosine + O2 + AH2
-
 Homo sapiens L-dopa + H2O + A
-
 ?

Synonyms

Synonyms Comment Organism
monophenol, dihydroxyphenylalanine:oxygen oxidoreductase copper binding metalloenzyme Homo sapiens
tyrosinase plays a core role in melanogenesis Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
17
-
 Cl- human tyrosinase source TXM13 pigment cell Homo sapiens
38.9
-
 Cl- human tyrosinase source HEK293-TYR Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
155
-
 human tyrosinase source TXM13 pigment cell Homo sapiens Cl-
164
-
 human tyrosinase source HEK293-TYR Homo sapiens Cl-