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Literature summary for 1.14.18.1 extracted from

  • McMahon, A.M.; Doyle, E.M.; Brooks, S.; OConnor, K.E.
    Biochemical characterization of the coexisting tyrosinase and laccase in the soil bacterium Pseudomonas putida F6 (2007), Enzyme Microb. Technol., 40, 1435-1441.
No PubMed abstract available
Search for more literature for 1.14.18.1

General Stability

General Stability Organism
monophenolase and diphenolase activities of tyrosinase are relatively stable across a broad range of pH values with maximum stability at pH 5.0 and 4.0. Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.23
-
 L-tyrosine
-
 Pseudomonas putida
0.33
-
 L-Dopa
-
 Pseudomonas putida
1.12
-
 alpha-methyl-DL-tyrosine
-
 Pseudomonas putida
3.07
-
 alpha-methyl-L-tyrosine
-
 Pseudomonas putida

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Pseudomonas putida 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
copper
-
 Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 determination by gel filtration chromatography Pseudomonas putida
39000
-
 determination by SDS-PAGE Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
 soil bacterium. The coexistence of this tyrosinase with a laccase in Pseudomonas putida F6 is reported and both activities characterised
-
Pseudomonas putida F6
-
 soil bacterium. The coexistence of this tyrosinase with a laccase in Pseudomonas putida F6 is reported and both activities characterised
-

Purification (Commentary)

Purification (Comment) Organism
combination of anion exchange chromatography, gel filtration and gel slicing Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.02912
-
 CE, purification, monophenolase activity Pseudomonas putida
0.05512
-
 CE, purification, diphenolase activity Pseudomonas putida
0.375
-
 DEAE-sepharose, purification of Pseudomonas putida F6 tyrosinase, monophenolase activity Pseudomonas putida
0.8125
-
 DEAE-sepharose, purification, diphenolase activity Pseudomonas putida
2.143
-
 mono Q, specific activity (units/mg) purification, monophenolase activity Pseudomonas putida
2.964
-
 gel filtration, purification, monophenolase activity Pseudomonas putida
3.058
-
 gel slice, purification, monophenolase activity Pseudomonas putida
4.725
-
 mono Q, purification, diphenolase activity Pseudomonas putida
4.818
-
 gel filtration, purification, diphenolase activity Pseudomonas putida
5.463
-
 gel slice, purification, diphenolase activity Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-methyl-DL-tyrosine + O2 + AH2
-
 Pseudomonas putida N-methyl-DL-dopa + H2O + A
-
 ?
alpha-methyl-DL-tyrosine + O2 + AH2
-
 Pseudomonas putida F6 N-methyl-DL-dopa + H2O + A
-
 ?
alpha-methyl-L-tyrosine + O2 + AH2
-
 Pseudomonas putida N-methyl-L-dopa + H2O + A
-
 ?
L-dopa + 1/2 O2 1.5fold higher affinity for L-tyrosine compared to L-dopa Pseudomonas putida L-dopaquinone + H2O
-
 ?
L-dopa + 1/2 O2 1.5fold higher affinity for L-tyrosine compared to L-dopa Pseudomonas putida F6 L-dopaquinone + H2O
-
 ?
L-tyrosine + O2 + AH2 1.5fold higher affinity for L-tyrosine compared to L-dopa Pseudomonas putida L-dopa + H2O + A
-
 ?
L-tyrosine + O2 + AH2 1.5fold higher affinity for L-tyrosine compared to L-dopa Pseudomonas putida F6 L-dopa + H2O + A
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 36000-39000 SDS-PAGE Pseudomonas putida

Synonyms

Synonyms Comment Organism
tyrosinase
-
 Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 stable over a broad range of temperatures and are most stable at 30°C Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Pseudomonas putida