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Literature summary for 1.14.18.1 extracted from

  • Munoz-Munoz, J.L.; Garcia-Molina, F.; Varon, R.; Rodriguez-Lopez, J.N.; Garcia-Canovas, F.; Tudela, J.
    Kinetic characterization of the oxidation of esculetin by polyphenol oxidase and peroxidase (2007), Biosci. Biotechnol. Biochem., 71, 390-396.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
O2 (KM as a kinetic constant) of the PPO/O2/ESC system at pH 7 Agaricus bisporus
0.041
-
O2 (KM as a kinetic constant) of the PPO/O2/ESC system at pH 4.5 Agaricus bisporus
0.63
-
esculetin (KM as a kinetic constant) of the PPO/O2/ESC system at pH 7 Agaricus bisporus
0.9
-
esculetin (KM as a kinetic constant) of the PPO/O2/ESC system at pH 4.5 Agaricus bisporus

Organism

Organism UniProt Comment Textmining
Agaricus bisporus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by Duckworth and Coleman's procedure, but with two additional chromatographic steps Agaricus bisporus

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Agaricus bisporus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1530
-
-
Agaricus bisporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
esculetin + 1/2 O2 demonstration, that esculetin is no inhibitor, but a substrate of mushroom polyphenol oxidase (PPO) and horseradish peroxidase (POD) Agaricus bisporus 2H-chromene-2,6,7-trione + H2O
-
?
L-dopa + 1/2 O2
-
Agaricus bisporus L-dopaquinone + H2O
-
?
L-tyrosine + O2 + AH2
-
Agaricus bisporus L-dopa + H2O + A
-
?

Synonyms

Synonyms Comment Organism
polyphenol oxidase
-
Agaricus bisporus
PPO
-
Agaricus bisporus
tyrosinase
-
Agaricus bisporus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
551
-
esculetin (kcat as a kinetic constant) of the PPO/O2/ESC system at pH 4.5 Agaricus bisporus
928
-
esculetin (kcat as a kinetic constant) of the PPO/O2/ESC system at pH 7 Agaricus bisporus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
for polyphenol oxidase (PPO) Agaricus bisporus

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7 assay at pH 4.5.and 7.0 Agaricus bisporus