KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
O2 | (KM as a kinetic constant) of the PPO/O2/ESC system at pH 7 | Agaricus bisporus | |
0.041 | - |
O2 | (KM as a kinetic constant) of the PPO/O2/ESC system at pH 4.5 | Agaricus bisporus | |
0.63 | - |
esculetin | (KM as a kinetic constant) of the PPO/O2/ESC system at pH 7 | Agaricus bisporus | |
0.9 | - |
esculetin | (KM as a kinetic constant) of the PPO/O2/ESC system at pH 4.5 | Agaricus bisporus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agaricus bisporus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by Duckworth and Coleman's procedure, but with two additional chromatographic steps | Agaricus bisporus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Agaricus bisporus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1530 | - |
- |
Agaricus bisporus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
esculetin + 1/2 O2 | demonstration, that esculetin is no inhibitor, but a substrate of mushroom polyphenol oxidase (PPO) and horseradish peroxidase (POD) | Agaricus bisporus | 2H-chromene-2,6,7-trione + H2O | - |
? | |
L-dopa + 1/2 O2 | - |
Agaricus bisporus | L-dopaquinone + H2O | - |
? | |
L-tyrosine + O2 + AH2 | - |
Agaricus bisporus | L-dopa + H2O + A | - |
? |
Synonyms | Comment | Organism |
---|---|---|
polyphenol oxidase | - |
Agaricus bisporus |
PPO | - |
Agaricus bisporus |
tyrosinase | - |
Agaricus bisporus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
551 | - |
esculetin | (kcat as a kinetic constant) of the PPO/O2/ESC system at pH 4.5 | Agaricus bisporus | |
928 | - |
esculetin | (kcat as a kinetic constant) of the PPO/O2/ESC system at pH 7 | Agaricus bisporus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
for polyphenol oxidase (PPO) | Agaricus bisporus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 7 | assay at pH 4.5.and 7.0 | Agaricus bisporus |