Cloned (Comment) | Organism |
---|---|
overproduction of apotyrosinase (TYRC) and its activator protein (ARF378) in Escherichia coli BL21(DE3)-pLysS. tyrC and arf378 are independently but not polycistronically placed under the control of a T7 promoter in a vector, pET-21a(+). His6-tagged TYRC and His6-tagged ARF378 are simultaneously overproduced in an Escherichia coli strain harboring the plasmid pET-mel2 | Streptomyces castaneoglobisporus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.1 | - |
L-Dopa | His6-tagged TYRC | Streptomyces castaneoglobisporus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | the activator protein ORF378 may facilitate the incorporation of Cu(II) into apotyrosinase | Streptomyces castaneoglobisporus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces castaneoglobisporus | - |
HUT6202 | - |
Streptomyces castaneoglobisporus HUT6202 | - |
HUT6202 | - |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces castaneoglobisporus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.1 | - |
- |
Streptomyces castaneoglobisporus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-dopa + 1/2 O2 | - |
Streptomyces castaneoglobisporus | L-dopaquinone + H2O | - |
? | |
L-dopa + 1/2 O2 | - |
Streptomyces castaneoglobisporus HUT6202 | L-dopaquinone + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
tyrosinase | - |
Streptomyces castaneoglobisporus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
880 | - |
L-Dopa | His6-tagged TYRC | Streptomyces castaneoglobisporus |