Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Trypsin | the tyrosinase activity of ST94 is enhanced to about 4times by proteolysis with trypsin to form a protein, termed ST94t | Illex argentinus |
Cloned (Comment) | Organism |
---|---|
two distinct messages for the squid tyrosinase, represented as cDNA-1 and cDNA-2 are expressed in the ink sac. The subunit of ST94 is synthesized as a premature protein with 625 amino acid residues and an 18-residue signal sequence regionis eliminated to form the mature subunit comprised of 607 amino acid residues with a deduced molecular mass of 68993 Da | Illex argentinus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
kojic acid | IC50: 0.048 mM for ST94 and ST94t; IC50: 0.048 mM for ST94 and ST94t | Illex argentinus | |
Phenylthiourea | IC50: 0.00017 mM for ST94, 0.00018 mM for ST94t; IC50: 0.00017 mM for ST94, 0.00018 mM for ST94t | Illex argentinus | |
tropolone | IC50: 0.0021 mM for ST94, 0.0022 mM for ST94t; IC50: 0.0021 mM for ST94, 0.0022 mM for ST94t | Illex argentinus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
tyramine | pH 6.8, 25°C, ST94t | Illex argentinus | |
0.24 | - |
D-Tyr | pH 6.8, 25°C, ST94t | Illex argentinus | |
0.24 | - |
3,4-Dihydroxyphenylpropionic acid | pH 6.8, 25°C, ST94t | Illex argentinus | |
0.32 | - |
tyramine | pH 6.8, 25°C, ST94 | Illex argentinus | |
0.32 | - |
D-Tyr | pH 6.8, 25°C, ST94 | Illex argentinus | |
0.35 | - |
L-Tyr | pH 6.8, 25°C, ST94t | Illex argentinus | |
0.39 | - |
dopamine | pH 6.8, 25°C, ST94t | Illex argentinus | |
0.49 | - |
3,4-Dihydroxyphenylpropionic acid | pH 6.8, 25°C, ST94 | Illex argentinus | |
0.57 | - |
L-Tyr | pH 6.8, 25°C, ST94 | Illex argentinus | |
1.3 | - |
dopamine | pH 6.8, 25°C, ST94 | Illex argentinus | |
2.7 | - |
D-Dopa | pH 6.8, 25°C, ST94t | Illex argentinus | |
3.2 | - |
D-Dopa | pH 6.8, 25°C, ST94 | Illex argentinus | |
6.5 | - |
L-Dopa | pH 6.8, 25°C, ST94t | Illex argentinus | |
6.8 | - |
L-Dopa | pH 6.8, 25°C, ST94 | Illex argentinus | |
7.3 | - |
pyrocatechol | pH 6.8, 25°C, ST94t | Illex argentinus | |
9.3 | - |
pyrocatechol | pH 6.8, 25°C, ST94 | Illex argentinus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | contains 2 copper atoms per subunit | Illex argentinus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63800 | - |
2 * 63800, ST94t | Illex argentinus |
70000 | - |
2 * 70000, ST94, SDS-PAGE | Illex argentinus |
127600 | - |
St94t | Illex argentinus |
140200 | - |
ST94, MALDI-TOF mass spectrometry | Illex argentinus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Illex argentinus | Q76DT9 | tyrosinase precursor 2 | - |
Illex argentinus | Q76DU0 | tyrosinase precursor 1 | - |
Purification (Comment) | Organism |
---|---|
- |
Illex argentinus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
ink | - |
Illex argentinus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxyphenylpropionic acid + O2 | - |
Illex argentinus | 3-(3,4-dioxocyclohexa-1,5-dien-1-yl)propionic acid | - |
? | |
D-dopa + 1/2 O2 | - |
Illex argentinus | D-dopaquinone + H2O | - |
? | |
D-Tyr + O2 | - |
Illex argentinus | D-dopa + H2O | - |
? | |
dopamine + 1/2 O2 | - |
Illex argentinus | dopamine quinone + H2O | - |
? | |
L-dopa + 1/2 O2 | - |
Illex argentinus | L-dopaquinone + H2O | - |
? | |
L-Tyr + O2 + AH2 | - |
Illex argentinus | L-dopa + H2O + A | - |
? | |
pyrocatechol + O2 | - |
Illex argentinus | ? | - |
? | |
tyramine + O2 | - |
Illex argentinus | 4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 63800, ST94t | Illex argentinus |
dimer | 2 * 70000, ST94, SDS-PAGE | Illex argentinus |
Synonyms | Comment | Organism |
---|---|---|
ST94 | - |
Illex argentinus |
ST94t | the tyrosinase activity of ST94 is enhanced by proteolysis with trypsin to form a protein, termed ST94t | Illex argentinus |
tyrosinase | - |
Illex argentinus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
pH 7.4, 20 min, stable up to | Illex argentinus |
50 | - |
pH 7.4, 20 min, about 20% loss of activity | Illex argentinus |
55 | - |
pH 7.4, 20 min, about 40% loss of activity | Illex argentinus |
60 | - |
pH 7.4, 20 min, about 80% loss of activity | Illex argentinus |
70 | - |
pH 7.4, 20 min, complete inactivation | Illex argentinus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Illex argentinus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6.0: about 55% of maximal activity, pH 9.0: about 45% of maximal activity | Illex argentinus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 11 | 4°C, 24 h, less than 10% loss of activity | Illex argentinus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Illex argentinus | isoelectric focusing | - |
4.1 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00017 | - |
IC50: 0.00017 mM for ST94, 0.00018 mM for ST94t | Illex argentinus | Phenylthiourea | |
0.0021 | - |
IC50: 0.0021 mM for ST94, 0.0022 mM for ST94t | Illex argentinus | tropolone | |
0.048 | - |
IC50: 0.048 mM for ST94 and ST94t | Illex argentinus | kojic acid |