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Literature summary for 1.14.18.1 extracted from

  • Naraoka, T.; Uchisawa, H.; Mori, H.; Matsue, H.; Chiba, S.; Kimura, A.
    Purification, characterization and molecular cloning of tyrosinase from the cephalopod mollusk,Illex argentinus (2003), Eur. J. Biochem., 270, 4026-4038.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Trypsin the tyrosinase activity of ST94 is enhanced to about 4times by proteolysis with trypsin to form a protein, termed ST94t Illex argentinus

Cloned(Commentary)

Cloned (Comment) Organism
two distinct messages for the squid tyrosinase, represented as cDNA-1 and cDNA-2 are expressed in the ink sac. The subunit of ST94 is synthesized as a premature protein with 625 amino acid residues and an 18-residue signal sequence regionis eliminated to form the mature subunit comprised of 607 amino acid residues with a deduced molecular mass of 68993 Da Illex argentinus

Inhibitors

Inhibitors Comment Organism Structure
kojic acid IC50: 0.048 mM for ST94 and ST94t; IC50: 0.048 mM for ST94 and ST94t Illex argentinus
Phenylthiourea IC50: 0.00017 mM for ST94, 0.00018 mM for ST94t; IC50: 0.00017 mM for ST94, 0.00018 mM for ST94t Illex argentinus
tropolone IC50: 0.0021 mM for ST94, 0.0022 mM for ST94t; IC50: 0.0021 mM for ST94, 0.0022 mM for ST94t Illex argentinus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
tyramine pH 6.8, 25°C, ST94t Illex argentinus
0.24
-
D-Tyr pH 6.8, 25°C, ST94t Illex argentinus
0.24
-
3,4-Dihydroxyphenylpropionic acid pH 6.8, 25°C, ST94t Illex argentinus
0.32
-
tyramine pH 6.8, 25°C, ST94 Illex argentinus
0.32
-
D-Tyr pH 6.8, 25°C, ST94 Illex argentinus
0.35
-
L-Tyr pH 6.8, 25°C, ST94t Illex argentinus
0.39
-
dopamine pH 6.8, 25°C, ST94t Illex argentinus
0.49
-
3,4-Dihydroxyphenylpropionic acid pH 6.8, 25°C, ST94 Illex argentinus
0.57
-
L-Tyr pH 6.8, 25°C, ST94 Illex argentinus
1.3
-
dopamine pH 6.8, 25°C, ST94 Illex argentinus
2.7
-
D-Dopa pH 6.8, 25°C, ST94t Illex argentinus
3.2
-
D-Dopa pH 6.8, 25°C, ST94 Illex argentinus
6.5
-
L-Dopa pH 6.8, 25°C, ST94t Illex argentinus
6.8
-
L-Dopa pH 6.8, 25°C, ST94 Illex argentinus
7.3
-
pyrocatechol pH 6.8, 25°C, ST94t Illex argentinus
9.3
-
pyrocatechol pH 6.8, 25°C, ST94 Illex argentinus

Metals/Ions

Metals/Ions Comment Organism Structure
copper contains 2 copper atoms per subunit Illex argentinus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63800
-
2 * 63800, ST94t Illex argentinus
70000
-
2 * 70000, ST94, SDS-PAGE Illex argentinus
127600
-
St94t Illex argentinus
140200
-
ST94, MALDI-TOF mass spectrometry Illex argentinus

Organism

Organism UniProt Comment Textmining
Illex argentinus Q76DT9 tyrosinase precursor 2
-
Illex argentinus Q76DU0 tyrosinase precursor 1
-

Purification (Commentary)

Purification (Comment) Organism
-
Illex argentinus

Source Tissue

Source Tissue Comment Organism Textmining
ink
-
Illex argentinus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dihydroxyphenylpropionic acid + O2
-
Illex argentinus 3-(3,4-dioxocyclohexa-1,5-dien-1-yl)propionic acid
-
?
D-dopa + 1/2 O2
-
Illex argentinus D-dopaquinone + H2O
-
?
D-Tyr + O2
-
Illex argentinus D-dopa + H2O
-
?
dopamine + 1/2 O2
-
Illex argentinus dopamine quinone + H2O
-
?
L-dopa + 1/2 O2
-
Illex argentinus L-dopaquinone + H2O
-
?
L-Tyr + O2 + AH2
-
Illex argentinus L-dopa + H2O + A
-
?
pyrocatechol + O2
-
Illex argentinus ?
-
?
tyramine + O2
-
Illex argentinus 4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + H2O
-
?

Subunits

Subunits Comment Organism
dimer 2 * 63800, ST94t Illex argentinus
dimer 2 * 70000, ST94, SDS-PAGE Illex argentinus

Synonyms

Synonyms Comment Organism
ST94
-
Illex argentinus
ST94t the tyrosinase activity of ST94 is enhanced by proteolysis with trypsin to form a protein, termed ST94t Illex argentinus
tyrosinase
-
Illex argentinus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
pH 7.4, 20 min, stable up to Illex argentinus
50
-
pH 7.4, 20 min, about 20% loss of activity Illex argentinus
55
-
pH 7.4, 20 min, about 40% loss of activity Illex argentinus
60
-
pH 7.4, 20 min, about 80% loss of activity Illex argentinus
70
-
pH 7.4, 20 min, complete inactivation Illex argentinus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Illex argentinus

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 6.0: about 55% of maximal activity, pH 9.0: about 45% of maximal activity Illex argentinus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 11 4°C, 24 h, less than 10% loss of activity Illex argentinus

pI Value

Organism Comment pI Value Maximum pI Value
Illex argentinus isoelectric focusing
-
4.1

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00017
-
IC50: 0.00017 mM for ST94, 0.00018 mM for ST94t Illex argentinus Phenylthiourea
0.0021
-
IC50: 0.0021 mM for ST94, 0.0022 mM for ST94t Illex argentinus tropolone
0.048
-
IC50: 0.048 mM for ST94 and ST94t Illex argentinus kojic acid