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Literature summary for 1.14.18.1 extracted from
- Expression and characterization of human tyrosinase from a bacterial expression system (2000), Comp. Biochem. Physiol. B, 125, 563-569.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-hydroxy-2-(hydroxymethyl)-2H-pyran-4-one | 1.12 mM, 50% inhibition of recombinant enzyme | Homo sapiens |  |
| arbutin | 3.7 mM, 50% inhibition of recombinant enzyme | Homo sapiens | |
| glutathione | 0.01 mM, 50% inhibition of recombinant enzyme | Homo sapiens | |
| L-phenylalanine | 8.34 mM, 50% inhibition of recombinant enzyme | Homo sapiens | |
| Lactic acid | 3.73 mM, 50% inhibition of recombinant enzyme | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.17 | - |
L-tyrosine | recombinant enzyme | Homo sapiens | |
| 0.36 | - |
L-Dopa | recombinant enzyme | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
x * 66000, recombinant enzyme, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme, DEAE-Sephacel, Ni2+-affinity column | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 26 | - |
recombinant enzyme | Homo sapiens |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 66000, recombinant enzyme, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
L-dopa oxidation, recombinant enzyme | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | less than 50% activity below pH 6.5 and above pH 8.5, L-dopa oxidation, recombinant enzyme | Homo sapiens |
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