Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-hydroxy-2-(hydroxymethyl)-2H-pyran-4-one | trivial name kojic acid, 1 mM, complete inhibition of L-dopa oxidation, 94% inhibition of tyrosinase activity | Streptomyces sp. | |
diethyldithiocarbamate | 1 mM, complete inhibition of L-dopa oxidation, 21% inhibition of tyrosinase activity | Streptomyces sp. | |
Hg2+ | inhibition of tyrosine hydroxylation | Streptomyces sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.69 | - |
phenol | - |
Streptomyces sp. | |
1.11 | - |
pyrogallol | - |
Streptomyces sp. | |
3 | - |
tyrosine | - |
Streptomyces sp. | |
4 | - |
Dopa | - |
Streptomyces sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | less than 3% intracellular activity | Streptomyces sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | 1 gatom per mol | Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | 36000 | gel filtration, SDS-PAGE | Streptomyces sp. |
36000 | - |
gel filtration | Streptomyces sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | - |
KY-453, isolated from soil | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, CM-cellulose, Sephadex G-100 | Streptomyces sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
215 | - |
- |
Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dopa + 1/2 O2 | - |
Streptomyces sp. | dopaquinone + H2O | - |
? | |
L-tyrosine + O2 + AH2 | - |
Streptomyces sp. | L-dopa + H2O + A | - |
? | |
phenol + O2 + AH2 | - |
Streptomyces sp. | o-dihydroxybenzene + H2O + A | - |
? | |
phenol + O2 + AH2 | - |
Streptomyces sp. | catechol + H2O + A | - |
? | |
pyrogallol + O2 | - |
Streptomyces sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 29000-36000, SDS-PAGE | Streptomyces sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
with L-dopa | Streptomyces sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.2 | - |
phenol | - |
Streptomyces sp. | |
7.2 | - |
L-tyrosine | - |
Streptomyces sp. | |
9.7 | - |
pyrogallol | - |
Streptomyces sp. | |
118 | - |
L-Dopa | - |
Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
with L-dopa | Streptomyces sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | 80% activity after 1 h incubation | Streptomyces sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00034 | - |
diethyldithiocarbamate | non-competitive vs. L-dopa | Streptomyces sp. | |
0.0062 | - |
diethyldithiocarbamate | non-competitive vs. L-tyrosine | Streptomyces sp. | |
0.013 | - |
5-hydroxy-2-(hydroxymethyl)-2H-pyran-4-one | non-competitive vs. L-dopa | Streptomyces sp. | |
0.057 | - |
5-hydroxy-2-(hydroxymethyl)-2H-pyran-4-one | non-competitive vs. L-tyrosine | Streptomyces sp. |