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Literature summary for 1.14.18.1 extracted from

  • Bru, R.; Sanchez-Ferrer, A.; Garcia-Carmona, F.
    Characteristics of tyrosinase in AOT-isooctane reverse micelles (1989), Biotechnol. Bioeng., 34, 304-308.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
4-methylcatechol
-
Mushroom

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
4-methylcatechol
-
Mushroom
0.17
-
4-methylcatechol in H2O Mushroom
0.2
-
4-methylcatechol measured in bis(2-ethylhexyl)sodium sulfosuccinate-isooctane reverse micelles Mushroom

Organism

Organism UniProt Comment Textmining
Mushroom
-
obtained from Sigma
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Mushroom
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylcatechol + O2
-
Mushroom 4-methyl-o-benzoquinone + H2O
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
in bis(2-ethylhexyl)sodium sulfosuccinate-isooctane reverse micelles Mushroom
45
-
in H2O Mushroom

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
free enzyme Mushroom

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7.5 pH 4.5: about 45% of maximal activity, pH 7.5: about 60% of maximal activity, free enzyme Mushroom
5 9 pH 5.0: about 30% of maximal activity, pH 9.0: about 50% of maximal activity, enzyme in reversed micelles Mushroom

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.23
-
4-methylcatechol in bis(2-ethylhexyl)sodium sulfosuccinate-isooctane reverse micelles Mushroom
3.6
-
4-methylcatechol in H2O, substrate inhibition Mushroom