Literature summary for 1.14.18.1 extracted from

Janovitz-Klapp, A.; Richard, F.; Nicholas, J.
Polyphenoloxidase from apple, partial purification and some properties (1989), Phytochemistry, 28, 2903-2907.

No PubMed abstract available

Search for more literature for 1.14.18.1

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4.1	-	chlorogenic acid	at pH 4.0, slight increase in Km above pH 5.0	Malus sp.
4.9	-	chlorogenic acid	at pH 5.5, slight increase in Km above pH 5.0	Malus sp.
5.2	-	4-methylcatechol	at pH 3.5-5.0, strong increase in Km above pH 5.0	Malus sp.
6.1	-	(+)-catechin	at pH 4.0, slight increase in Km above pH 4.0	Malus sp.
6.6	-	(+)-catechin	at pH 5.5, slight increase in Km above pH 4.0	Malus sp.
20.5	-	4-methylcatechol	at pH 6.3, strong increase in Km above pH 5.0	Malus sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
46000	-	gel filtration	Malus sp.

Organism

Organism	UniProt	Comment	Textmining
Malus sp.	-	apple, var. red delicious	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate, Phenyl Sepharose	Malus sp.

Source Tissue

Source Tissue	Comment	Organism	Textmining
fruit	peel and cortex	Malus sp.	-
peel	-	Malus sp.	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
11330	-	-	Malus sp.

Storage Stability

Storage Stability	Organism
4°C, 15 days, 10% loss of activity	Malus sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylcatechol + O2	-	Malus sp.	4-methyl-o-benzoquinone + H2O	-	?
chlorogenic acid + O2	-	Malus sp.	chlorogenoquinone + H2O	-	?
D-catechin + O2	-	Malus sp.	?	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
30	-	4 h, pH 4.5, 50% loss of activity	Malus sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.5	5	with 4-methylcatechol, chlorogenic acid and (+)-catechin	Malus sp.

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Release 2023.1 (January 2023)