Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | 12 mM, 50% inhibition | Berberis stolonifera | |
Bathocuproine | - |
Berberis stolonifera | |
CN- | 1 mM, complete inhibition | Berberis stolonifera | |
diethyldithiocarbamate | 0.3 mM, complete inhibition | Berberis stolonifera | |
dipicolinic acid | - |
Berberis stolonifera | |
EDTA | no inhibition EDTA | Berberis stolonifera | |
Thiourea | 13 mM, 50% inhibition | Berberis stolonifera |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
2 * 36000, SDS-PAGE | Berberis stolonifera |
60000 | - |
gel filtration | Berberis stolonifera |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Berberis stolonifera | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Sephadex G-25, hydoroxylapatite-DEAE-Sephacel, Q Sepharose, Mono Q, isoelectric focusing | Berberis stolonifera |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.065 | - |
with tyrosine | Berberis stolonifera |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-coclaurine + ascorbate + O2 | - |
Berberis stolonifera | (R)-3-hydroxycoclaurine + dehydroascorbate + H2O | - |
? | |
(S)-coclaurine + ascorbate + O2 | - |
Berberis stolonifera | (S)-3-hydroxycoclaurine + dehydroascorbate + H2O | - |
? | |
4-methylcatechol + ascorbate + O2 | - |
Berberis stolonifera | 4-methyl-o-benzoquinone + dehydroascorbate + H2O | - |
? | |
L-tyrosine + ascorbate + O2 | - |
Berberis stolonifera | L-dopa + dehydroascorbate + H2O | - |
? | |
N-methylcoclaurine + ascorbate + O2 | - |
Berberis stolonifera | 3-hydroxy-N-methylcoclaurine + dehydroascorbate + H2O | - |
? | |
tyramine + ascorbate + O2 | - |
Berberis stolonifera | 4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + dehydroascorbate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 36000, SDS-PAGE | Berberis stolonifera |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 30 | - |
Berberis stolonifera |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Berberis stolonifera |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 7.5 | 50% of maximal activity at pH 4.5 and pH 7.5 | Berberis stolonifera |