Protein Variants | Comment | Organism |
---|---|---|
R244A | enzymic activity about 20% of wild-type | Malus domestica |
R244G | enzymic activity about 20% of wild-type | Malus domestica |
R244K | enzymic activity about 20% of wild-type | Malus domestica |
S246A | enzymic activity about 50% of wild-type | Malus domestica |
S246F | enzymic activity less than 5% of wild-type | Malus domestica |
S246G | enzymic activity about 50% of wild-type | Malus domestica |
S246T | enzymic activity less than 5% of wild-type | Malus domestica |
S246Y | enzymic activity less than 5% of wild-type | Malus domestica |
S257A | KM-value similar to wild-type | Malus domestica |
T157A | enzymic activity about 40% of wild-type | Malus domestica |
V159G | enzymic activity about 30% of wild-type | Malus domestica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
ascorbate | mutant S246A, pH 7.4, 30°C | Malus domestica | |
0.34 | - |
ascorbate | mutant E109D, pH 7.4, 30°C | Malus domestica | |
0.36 | - |
ascorbate | mutant S257A, pH 7.4, 30°C | Malus domestica | |
0.38 | - |
ascorbate | wild-type, pH 7.4, 30°C | Malus domestica | |
0.82 | - |
ascorbate | mutant T157A, pH 7.4, 30°C | Malus domestica | |
0.82 | - |
ascorbate | mutant V159G, pH 7.4, 30°C | Malus domestica | |
0.92 | - |
ascorbate | mutant S246G, pH 7.4, 30°C | Malus domestica | |
0.99 | - |
ascorbate | mutant R244A, pH 7.4, 30°C | Malus domestica | |
1.2 | - |
ascorbate | mutant R244K, pH 7.4, 30°C | Malus domestica | |
1.25 | - |
ascorbate | mutant R244G, pH 7.4, 30°C | Malus domestica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | together with cosubstrate ascorbate, located in 2-His-1-carboxylate facial triad binding pocket | Malus domestica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Malus domestica | - |
Borkh. | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
1-aminocyclopropane-1-carboxylate + ascorbate + O2 = ethene + cyanide + dehydroascorbate + CO2 + 2 H2O | modelling studies, catalytic mechanism | Malus domestica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-amino-2-ethylcyclopropane-1-carboxylate + L-ascorbate + O2 + HCO3- | - |
Malus domestica | 1-butene + cyanide + dehydroascorbate + CO2 + H2O | - |
? |