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Literature summary for 1.14.17.4 extracted from
- Steady-state kinetics of substrate binding and iron release in tomato ACC oxidase (2001), Biochemistry, 40, 9717-9724.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | required | Solanum lycopersicum |  |
| CO2 | activates | Solanum lycopersicum | |
| additional information | no activation by bisulfite, isozyme AOC1 | Solanum lycopersicum | |
| O2 | required | Solanum lycopersicum | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| isozyme ACO1, overexpression in Escherichia coli BL21(DE3)-pLysS | Solanum lycopersicum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-aminocyclopropane-1-carboxylate | slight inhibition at high concentration | Solanum lycopersicum | |
| Zn2+ | competitive to Fe2+ | Solanum lycopersicum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.028 | - |
O2 | recombinant isozyme ACO1, in presence of 0.5 mM L-ascorbate and 20 mM CO2/HCO3-, pH 7.2, 25°C | Solanum lycopersicum | |
| 0.077 | - |
1-aminocyclopropane-1-carboxylate | recombinant isozyme ACO1, in presence of 0.5 mM L-ascorbate and 20 mM CO2/HCO3-, pH 7.2, 25°C | Solanum lycopersicum | |
| 4.6 | - |
L-ascorbate | recombinant isozyme ACO1, in presence of 0.5 mM L-ascorbate and 20 mM CO2/HCO3-, pH 7.2, 25°C | Solanum lycopersicum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | required | Solanum lycopersicum | |
| Fe2+ | a single bound Fe2+ per monomer | Solanum lycopersicum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-aminocyclopropane-1-carboxylate + ascorbate + O2 | Solanum lycopersicum | catalyses the final step in ethylene biosynthesis | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum lycopersicum | - |
isozyme ACO1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant isozyme ACO1 from Escherichia coli BL21(DE3)-pLysS, to near homogeneity | Solanum lycopersicum |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 1-aminocyclopropane-1-carboxylate + ascorbate + O2 = ethene + cyanide + dehydroascorbate + CO2 + 2 H2O | catalytic mechanism | Solanum lycopersicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-aminocyclopropane-1-carboxylate + ascorbate + O2 | catalyses the final step in ethylene biosynthesis | Solanum lycopersicum | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
| 1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 | specific for the substrates | Solanum lycopersicum | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
| 1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 | i.e. ACC | Solanum lycopersicum | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Solanum lycopersicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Solanum lycopersicum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 38.9 | - |
1-aminocyclopropane-1-carboxylate | recombinant isozyme ACO1, in presence of 5 mM L-ascorbate and 20 mM CO2/HCO3-, pH 7.2, 25°C | Solanum lycopersicum | |
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