Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Malus domestica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-aminoisobutyric acid | competitive to 1-aminocyclopropane-1-carboxylate | Malus domestica | |
D-alanine | competitive to 1-aminocyclopropane-1-carboxylate | Malus domestica | |
D-alpha-aminobutyric acid | competitive to 1-aminocyclopropane-1-carboxylate | Malus domestica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
1-aminocyclopropane-1-carboxylate | recombinant enzyme, pH 7.0, 30°C | Malus domestica | |
14.7 | - |
alpha-aminoisobutyrate | recombinant enzyme, pH 7.0, 30°C | Malus domestica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-aminocyclopropane-1-carboxylate + ascorbate + O2 | Malus domestica | - |
ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Malus domestica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli | Malus domestica |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
1-aminocyclopropane-1-carboxylate + ascorbate + O2 = ethene + cyanide + dehydroascorbate + CO2 + 2 H2O | catalytic mechanism | Malus domestica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.003 | - |
at 10 mM L-alpha-aminobutyric acid, purified recombinant enzyme | Malus domestica |
0.008 | - |
at 10 mM L-alanine, purified recombinant enzyme | Malus domestica |
0.023 | - |
at 10 mM D-alanine, purified recombinant enzyme | Malus domestica |
0.051 | - |
at 10 mM alpha-aminoisobutyric acid, purified recombinant enzyme | Malus domestica |
0.059 | - |
at 10 mM D-alpha-aminobutyric acid, purified recombinant enzyme | Malus domestica |
0.13 | - |
at 10 mM 1-aminocyclopropane-1-carboxylate, purified recombinant enzyme | Malus domestica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-aminocyclopropane-1-carboxylate + ascorbate + O2 | - |
Malus domestica | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 | stereoselectivity | Malus domestica | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 | i.e. ACC | Malus domestica | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 | N-hydroxyl-1-aminocyclopropane-1-carboxylate is no intermediate | Malus domestica | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
alpha-aminoisobutyric acid + L-ascorbate + O2 | - |
Malus domestica | ammonia + acetone + dehydroascorbate + CO2 + H2O | - |
? | |
D-alanine + L-ascorbate + O2 | - |
Malus domestica | ? | - |
? | |
D-alpha-aminobutyric acid + L-ascorbate + O2 | - |
Malus domestica | ammonia + acetone + dehydroascorbate + CO2 + H2O | - |
? | |
L-alanine + L-ascorbate + O2 | - |
Malus domestica | ? | - |
? | |
L-alpha-aminobutyric acid + L-ascorbate + O2 | - |
Malus domestica | ammonia + acetone + dehydroascorbate + CO2 + H2O | - |
? | |
N-hydroxy-alpha-aminoisobutyric acid + L-ascorbate + O2 | - |
Malus domestica | ammonia + acetone + dehydroascorbate + CO2 + H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Malus domestica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
1-aminocyclopropane-1-carboxylate | recombinant enzyme, pH 7.0, 30°C | Malus domestica | |
0.07 | - |
alpha-aminoisobutyrate | recombinant enzyme, pH 7.0, 30°C | Malus domestica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Malus domestica |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
4.2 | - |
D-alpha-aminobutyric acid | pH 7.0, 30°C | Malus domestica | |
7.5 | - |
alpha-aminoisobutyric acid | pH 7.0, 30°C | Malus domestica | |
28 | - |
D-alanine | pH7.0, 30°C | Malus domestica |