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Literature summary for 1.14.17.4 extracted from

  • Zhang, Z.; Schofield, C.J.; Baldwin, J.E.; Thomas, P.; John, P.
    Expression, purification and characterization of 1-aminocyclopropane-1-carboxylate oxidase from tomato in Escherichia coli (1995), Biochem. J., 307, 77-85.
No PubMed abstract available
Search for more literature for 1.14.17.4

Activating Compound

Activating Compound Comment Organism Structure
ascorbate required Solanum lycopersicum
ascorbate cannot be replaced by DTT or NADH Solanum lycopersicum
bovine serum albumin stimulates the recombinant enzyme by 41% at 0.1 mM Solanum lycopersicum
catalase recombinant enzyme, stimulates by 36% at 0.5 mg/ml Solanum lycopersicum
CO2 requires HCO3-/CO2 mixture Solanum lycopersicum
dithiothreitol recombinant enzyme, stimulates by 38% at 1 mM Solanum lycopersicum
ethylene stimulates Solanum lycopersicum

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli BL21(DE3) Solanum lycopersicum

Inhibitors

Inhibitors Comment Organism Structure
diethyl dicarbonate complete inactivation at 1.5 mM, pH 6.8, after 20 min Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0053
-
 Fe2+ recombinant enzyme, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C Solanum lycopersicum
0.032
-
 1-aminocyclopropane-1-carboxylate recombinant enzyme, in presence of 5mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C Solanum lycopersicum
1
-
 5,6-O-isopropylidine L-ascorbate recombinant enzyme, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C Solanum lycopersicum
1.7
-
 D-ascorbate recombinant enzyme, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C Solanum lycopersicum
16.7
-
 L-ascorbate recombinant enzyme, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C Solanum lycopersicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35820
-
 electron spray mass spectrometry Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-aminocyclopropane-1-carboxylate + ascorbate + O2 Solanum lycopersicum
-
 ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from E. coli, 9.8fold to near homogeneity Solanum lycopersicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.035
-
 purified enzyme Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-amino-2-ethylcyclopropane-1-carboxylate + L-ascorbate + O2 prefers mixture of 1R,2S- and 1S,2R-amino-2-ethylcyclopropane-1-carboxylate, less effective with racemic mixture of 1R,2R- and 1S,2S-diastereomers Solanum lycopersicum 1-butene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?
1-aminocyclopropane-1-carboxylate + 5,6-O-isopropylidine L-ascorbate + O2 better cosubstrate than L-ascorbate Solanum lycopersicum ethylene + cyanide + 5,6-O-isopropylidine L-dehydroascorbate + CO2 + H2O
-
 ?
1-aminocyclopropane-1-carboxylate + ascorbate + O2
-
 Solanum lycopersicum ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?
1-aminocyclopropane-1-carboxylate + D-ascorbate + O2 better cosubstrate than L-ascorbate Solanum lycopersicum ethylene + cyanide + D-dehydroascorbate + CO2 + H2O
-
 ?
1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 i.e. ACC Solanum lycopersicum ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?
additional information L-galactono-gamma-lactone is no cosubstrate Solanum lycopersicum ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
 assay at Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Solanum lycopersicum