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Literature summary for 1.14.17.4 extracted from
- Purification and characterization of 1-aminocyclopropane-1-carboxylate oxidase from apple fruit (1992), Proc. Natl. Acad. Sci. USA, 89, 9789-9793.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | absolutely dependent on | Malus domestica |  |
| ascorbate | minimal 1 mM | Malus domestica | |
| CO2 | absolutely dependent on | Malus domestica | |
| CO2 | half-maximal activity at 0.5% atmospheric CO2 in vivo or 0.15 mM in the medium in vitro | Malus domestica | |
| dithiothreitol | activates in presence of ascorbate, but cannot replace ascorbate | Malus domestica | |
| ethylene | activation, in vivo and in vitro | Malus domestica | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Malus domestica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-aminoisobutyric acid | - |
Malus domestica | |
| Co2+ | - |
Malus domestica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | absolutely required | Malus domestica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
1 * 35000, SDS-PAGE | Malus domestica |
| 39000 | - |
gel filtration | Malus domestica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-aminocyclopropane-1-carboxylate + ascorbate + O2 | Malus domestica | absolutely dependent on CO2 | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Malus domestica | - |
cv. Golden Delicious | - |
| Malus domestica | - |
inducible by ethylene | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 180fold to near homogeneity | Malus domestica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | in climacteric stage | Malus domestica | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.02 | - |
purified enzyme | Malus domestica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-aminocyclopropane-1-carboxylate + ascorbate + O2 | absolutely dependent on CO2 | Malus domestica | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
| 1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 | absolutely dependent on CO2 | Malus domestica | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
| 1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 | i.e. ACC | Malus domestica | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
| 1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 | absolutely dependent on ascorbate | Malus domestica | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 35000, SDS-PAGE | Malus domestica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Malus domestica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Malus domestica |
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