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Literature summary for 1.14.17.3 extracted from

  • Kline, C.D.; Mayfield, M.; Blackburn, N.J.
    HHM motif at the CuH-site of peptidylglycine monooxygenase is a pH-dependent conformational switch (2013), Biochemistry, 52, 2586-2596.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.17.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in CHO DG44 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
H107A low activity Homo sapiens
H108A low activity Homo sapiens
M109I low activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0033
-
 dansyl-L-Tyr-L-Val-Gly mutant enzyme H107A, in100 mM MES pH 5.5, at 37°C Homo sapiens
0.0082
-
 dansyl-L-Tyr-L-Val-Gly wild type enzyme, in100 mM MES pH 5.5, at 37°C Homo sapiens
0.0118
-
 dansyl-L-Tyr-L-Val-Gly mutant enzyme M109I, in100 mM MES pH 5.5, at 37°C Homo sapiens
0.0194
-
 dansyl-L-Tyr-L-Val-Gly mutant enzyme H108A, in100 mM MES pH 5.5, at 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
copper the enzyme contains two mononuclear Cu centers Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
peptidylglycine + ascorbate + O2 Homo sapiens
-
 peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-L-Tyr-L-Val-Gly + ascorbate + O2
-
 Homo sapiens ?
-
 ?
dansyl-L-Tyr-L-Val-Gly + ascorbate + O2
-
 Homo sapiens ?
-
 ?
peptidylglycine + ascorbate + O2
-
 Homo sapiens peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?

Synonyms

Synonyms Comment Organism
peptidylglycine alpha-hydroxylating monooxygenase
-
 Homo sapiens
PHM
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.08
-
 dansyl-L-Tyr-L-Val-Gly mutant enzyme H107A, in100 mM MES pH 5.5, at 37°C Homo sapiens
0.11
-
 dansyl-L-Tyr-L-Val-Gly mutant enzyme H108A, in100 mM MES pH 5.5, at 37°C Homo sapiens
4.6
-
 dansyl-L-Tyr-L-Val-Gly mutant enzyme M109I, in100 mM MES pH 5.5, at 37°C Homo sapiens
13.8
-
 dansyl-L-Tyr-L-Val-Gly wild type enzyme, in100 mM MES pH 5.5, at 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
 the enzyme undergoes loss of activity at lower pHs due to a protonation Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ascorbate
-
 Homo sapiens