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Literature summary for 1.14.17.3 extracted from

  • Trendel, J.A.; Ellis, N.; Sarver, J.G.; Klis, W.A.; Dhananjeyan, M.; Bykowski, C.A.; Reese, M.D.; Erhardt, P.W.
    Catalytically active peptidylglycine alpha-amidating monooxygenase in the media of androgen-independent prostate cancer cell lines (2008), J. Biomol. Screen., 13, 804-809.
    View publication on PubMed
Search for more literature for 1.14.17.3

Inhibitors

Inhibitors Comment Organism Structure
4-phenylbut-3-enoic acid PBA Homo sapiens
diethyldithiocarbamate DDC Homo sapiens
Disulfiram
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0044
-
 Trinitrophenyl-D-Tyr-Val-Gly PC-3 TIS media Homo sapiens
0.0068
-
 Trinitrophenyl-D-Tyr-Val-Gly DU-145 TIS media Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-
extracellular
-
 Homo sapiens
-
-
membrane
-
 Homo sapiens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
copper
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 PAM, determined by SDS-PAGE and Western blot analysis Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens PHM replaces the pro-(S) hydrogen of the carboxy-terminal glycine with a hydroxyl group to form an alpha-hydroxyglycine intermediate ?
-
 ?
peptidylglycine + ascorbate + O2 Homo sapiens
-
 peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
cell extracts are prepared Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
CaP cell line
-
 Homo sapiens
-
DU-145 cell
-
 Homo sapiens
-
PC-3 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information PHM replaces the pro-(S) hydrogen of the carboxy-terminal glycine with a hydroxyl group to form an alpha-hydroxyglycine intermediate Homo sapiens ?
-
 ?
peptidylglycine + ascorbate + O2
-
 Homo sapiens peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?
trinitrophenyl-D-Tyr-Val-Gly + ascorbate + O2 activity assay Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
PAM PAM consists of O2-sensitive PHM and peptidyl-alpha-hydroxyglycine alpha-amidating lyase, PAL, activities Homo sapiens
peptidylglycine alpha-amidating monooxygenase
-
 Homo sapiens
peptidylglycine alpha-hydroxylating monooxygenase
-
 Homo sapiens
PHM
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 activity assay Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ascorbate
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00051
-
 Disulfiram DU-145 TIS media Homo sapiens
0.00065
-
 Disulfiram PC-3 TIS media Homo sapiens
0.00113
-
 diethyldithiocarbamate DU-145 TIS media Homo sapiens
0.00119
-
 diethyldithiocarbamate PC-3 TIS media Homo sapiens
0.0034
-
 4-phenylbut-3-enoic acid PC-3 TIS media Homo sapiens
0.0057
-
 4-phenylbut-3-enoic acid DU-145 TIS media Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000053
-
 PC-3 TIS media Homo sapiens diethyldithiocarbamate
0.000096
-
 PC-3 TIS media Homo sapiens 4-phenylbut-3-enoic acid
0.00011
-
 DU-145 TIS media Homo sapiens diethyldithiocarbamate
0.00068
-
 DU-145 TIS media Homo sapiens 4-phenylbut-3-enoic acid