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Literature summary for 1.14.17.3 extracted from

  • Evans, J.P.; Blackburn, N.J.; Klinman, J.P.
    The catalytic role of the copper ligand H172 of peptidylglycine alpha-hydroxylating monooxygenase: A kinetic study of the H172A mutant (2006), Biochemistry, 45, 15419-15429.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Cricetulus griseus

Protein Variants

Protein Variants Comment Organism
H172A coppper biniding similar to wild type, but showed a 1000fold decrease in turnover rate Cricetulus griseus

Inhibitors

Inhibitors Comment Organism Structure
ferrocyanide inhibiting above 0.6 mM Cricetulus griseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
ferrocyanide 4 mM alpha-hydroxyhippuric acid, 0.21 mM O2, 100 mM MES pH 6.0, 30 mM KCl, 1 microM CuSO4 Cricetulus griseus
0.26
-
ferrocyanide 40 mM alpha-hydroxyhippuric acid, 0.95 mM O2, 100 mM MES pH 6.0, 30 mM KCl, 1 microM CuSO4 Cricetulus griseus
0.292
-
alpha-hydroxyhippuric acid 0.4 mM ferrocyanide, 100 mM MES pH 6.0, 30 mM KCl, 1 microM CuSO4 Cricetulus griseus

Organism

Organism UniProt Comment Textmining
Cricetulus griseus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Cricetulus griseus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Cricetulus griseus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hippuric acid + ascorbate + O2
-
Cricetulus griseus alpha-hydroxyhippuric acid + dehydroascorbate + H2O
-
?
hippuric acid + ferrocyanide + O2
-
Cricetulus griseus alpha-hydroxyhippuric acid + ? + H2O
-
?

Synonyms

Synonyms Comment Organism
peptidylglycine alpha-hydroxylating monooxygenase
-
Cricetulus griseus
PHM
-
Cricetulus griseus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
51
-
alpha-hydroxyhippuric acid 0.4 mM ferrocyanide, 100 mM MES pH 6.0, 30 mM KCl, 1 microM CuSO4 Cricetulus griseus