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Literature summary for 1.14.17.3 extracted from

  • Miller, L.A.; Baumgart, L.E.; Chew, G.H.; deLong, M.A.; Galloway, L.C.; Jung, K.W.; Merkler, K.A.; Nagle, A.S.; Poore, D.D.; Yoon, C.H.; Merkler, D.J.
    Glutathione, S-substituted glutathiones, and leukotriene C4 as substrates for peptidylglycine alpha-amidating monooxygenase (2003), Arch. Biochem. Biophys., 412, 3-12.
    View publication on PubMed
Search for more literature for 1.14.17.3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 S-decyl-glutathione pH 6.0, 37°C Rattus norvegicus
0.027
-
 S-(4-nitrobenzyl)-glutathione pH 6.0, 37°C Rattus norvegicus
0.032
-
 S-octyl-glutathione pH 6.0, 37°C Rattus norvegicus
0.035
-
 leukotriene C4 pH 6.0, 37°C Rattus norvegicus
0.035
-
 S-hexyl-glutathione pH 6.0, 37°C Rattus norvegicus
0.067
-
 S-butyl-glutathione pH 6.0, 37°C Rattus norvegicus
0.1
-
 N-decanoylglycine pH 6.0, 37°C Rattus norvegicus
0.11
-
 S-propyl-glutathione pH 6.0, 37°C Rattus norvegicus
0.13
-
 glutathione pH 6.0, 37°C Rattus norvegicus
0.22
-
 D-Tyr-Val-Gly pH 6.0, 37°C Rattus norvegicus
0.23
-
 S-ethyl-glutathione pH 6.0, 37°C Rattus norvegicus
0.27
-
 S-methyl-glutathione pH 6.0, 37°C Rattus norvegicus
0.42
-
 N-hexanoylglycine pH 6.0, 37°C Rattus norvegicus
1.1
-
 S-(1,2-dicarboxyethyl)-glutathione pH 6.0, 37°C Rattus norvegicus
2.1
-
 gamma-Glu-Gly-Gly pH 6.0, 37°C Rattus norvegicus
9.3
-
 N-acetylglycine pH 6.0, 37°C Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ bifunctional copper- and zinc-dependent enzyme Rattus norvegicus
Zn2+ bifunctional copper- and zinc-dependent enzyme Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione + ascorbate + O2 Rattus norvegicus
-
 gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
-
 ?
additional information Rattus norvegicus cleavage of CN bond in N-acetylated glycines, R-CO-NH-CH2-COOH, e.g. found in neuropeptide prohormones, inhibition of the enzyme leads to a decrease in alpha-aminated peptide production and accumulation of glycine-extended precursors ?
-
 ?
R-CO-NH-CH2-COOH + ascorbate + O2 Rattus norvegicus
-
 R-CO-NH2 + CHO-COOH + dehydroascorbate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 bifunctional copper- and zinc-dependent enzyme
-

Reaction

Reaction Comment Organism Reaction ID
[peptide]-glycine + 2 ascorbate + O2 = [peptide]-(2S)-2-hydroxyglycine + 2 monodehydroascorbate + H2O cleavage of CN bond in N-acetylated glycines, R-CO-NH-CH2-COOH, e.g. found in neuropeptide prohormones Rattus norvegicus
a

Source Tissue

Source Tissue Comment Organism Textmining
medullary thyroid carcinoma cell
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rattus norvegicus
3
-
 purified recombinant enzyme Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Tyr-Val-Gly + ascorbate + O2
-
 Rattus norvegicus D-Tyr-Val-NH2 + glyoxylate + dehydroascorbate + H2O
-
 ?
gamma-Glu-Gly-Gly + ascorbate + O2
-
 Rattus norvegicus gamma-Glu-Gly-NH2 + glyoxylate + dehydroascorbate + H2O
-
 ?
glutathione + ascorbate + O2
-
 Rattus norvegicus gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
-
 ?
leukotriene C4 + ascorbate + O2
-
 Rattus norvegicus ? + dehydroascorbate + H2O
-
 ?
additional information cleavage of CN bond in N-acetylated glycines, R-CO-NH-CH2-COOH, e.g. found in neuropeptide prohormones, inhibition of the enzyme leads to a decrease in alpha-aminated peptide production and accumulation of glycine-extended precursors Rattus norvegicus ?
-
 ?
additional information substrate specificity, cleavage of CN bond in N-acetylated glycines, R-CO-NH-CH2-COOH, e.g. found in neuropeptide prohormones Rattus norvegicus ?
-
 ?
N-acetylglycine + ascorbate + O2
-
 Rattus norvegicus acetylamine + glyoxylate + dehydroascorbate + H2O
-
 ?
N-decanoylglycine + ascorbate + O2
-
 Rattus norvegicus decanoylamine + glyoxylate + dehydroascorbate + H2O
-
 ?
N-hexanoylglycine + ascorbate + O2
-
 Rattus norvegicus hexanoylamine + glyoxylate + dehydroascorbate + H2O
-
 ?
R-CO-NH-CH2-COOH + ascorbate + O2
-
 Rattus norvegicus R-CO-NH2 + CHO-COOH + dehydroascorbate + H2O
-
 ?
R-CO-NH-CH2-COOH + ascorbate + O2 via hydroxylated reaction intermediates Rattus norvegicus R-CO-NH2 + CHO-COOH + dehydroascorbate + H2O
-
 ?
S-(1,2-dicarboxyethyl)-glutathione + ascorbate + O2
-
 Rattus norvegicus S-(1,2-dicarboxyethyl)-gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
-
 ?
S-(4-nitrobenzyl)-glutathione + ascorbate + O2
-
 Rattus norvegicus S-(4-nitrobenzyl)-gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
-
 ?
S-butyl-glutathione + ascorbate + O2
-
 Rattus norvegicus S-butyl-gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
-
 ?
S-decyl-glutathione + ascorbate + O2
-
 Rattus norvegicus S-decyl-gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
-
 ?
S-ethyl-glutathione + ascorbate + O2
-
 Rattus norvegicus S-ethyl-gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
-
 ?
S-hexyl-glutathione + ascorbate + O2
-
 Rattus norvegicus S-hexyl-gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
-
 ?
S-methyl-glutathione + ascorbate + O2
-
 Rattus norvegicus S-methyl-gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
-
 ?
S-octyl-glutathione + ascorbate + O2
-
 Rattus norvegicus S-octyl-gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
-
 ?
S-propyl-glutathione + ascorbate + O2
-
 Rattus norvegicus S-propyl-gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
-
 ?

Synonyms

Synonyms Comment Organism
PAM
-
 Rattus norvegicus
peptidylglycine alpha-amidating monooxygenase
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Rattus norvegicus