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Literature summary for 1.14.17.3 extracted from

  • Ping, D.; Mounier, C.E.; May, S.W.
    Reaction versus subsite stereospecificity of peptidylglycine alpha-monooxygenase and peptidylamidoglycolate lyase, the two enzymes involved in peptide amidation (1995), J. Biol. Chem., 270, 29250-29255.
    View publication on PubMed
Search for more literature for 1.14.17.3

Inhibitors

Inhibitors Comment Organism Structure
acetyl-D-Leu-OCH2COOH
-
 Bos taurus
acetyl-D-Phe-Gly
-
 Bos taurus
acetyl-D-Phe-OCH2COOH
-
 Bos taurus
acetyl-DL-Phe-OCH2COOH
-
 Bos taurus
acetyl-Gly-OCH2COOH
-
 Bos taurus
acetyl-L-Leu-OCH2COOH
-
 Bos taurus
acetyl-L-Phe-D-Phe-Gly
-
 Bos taurus
acetyl-L-Phe-Gly only substrate for alpha-hydroxylation Bos taurus
acetyl-L-Phe-OCH2COOH
-
 Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
peptidylglycine + ascorbate + O2 Bos taurus
-
 peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
pituitary gland
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information EC 1.14.17.3 is often called peptidylglycine alpha-amidating monooxygenase (PAM) and the alpha-amidated product is mentioned as the product of the reaction, but the alpha-amidation of glycine-extended peptides is a two-step process catalyzed by 2 enzymes: 1. EC 1.14.17.3: production of peptidyl(2-hydroxyglycine) by a copper, molecular oxygen and ascorbate-dependent peptidyl-glycine alpha-hydroxylating monooxygenase (PMH) and 2. conversion of the peptidyl-alpha-hydroxyglycine derivative into an alpha-amidated product at physiological pH by peptidyl-alpha-hydroxyglycine alpha-amidating lyase Bos taurus ?
-
 ?
additional information acetyl-L-Phe-Gly, acetyl-L-Phe-L-Phe-Gly, or (S)-O-acetyl-mandelyl-Gly, and stereoisomers are alpha-hydroxylated but not alpha-amidated Bos taurus ?
-
 ?
peptidylglycine + ascorbate + O2
-
 Bos taurus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?
peptidylglycine + ascorbate + O2 COOH-terminal glycine Bos taurus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
 ?

Synonyms

Synonyms Comment Organism
More EC 1.14.17.3 is often called peptidylglycine alpha-amidating monooxygenase (PAM) and the alpha-amidated product is mentioned as the product of the reaction, but the alpha-amidation of glycine-extended peptides is a two-step process catalyzed by 2 enzymes: 1. EC 1.14.17.3: production of peptidyl(2-hydroxyglycine) by a copper, molecular oxygen and ascorbate-dependent peptidyl-glycine alpha-hydroxylating monooxygenase (PHM), 2. conversion of the peptidyl-alpha-hydroxyglycine derivative into an alpha-amidated product at physiological pH by peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PHL) Bos taurus

Cofactor

Cofactor Comment Organism Structure
ascorbate
-
 Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.002
-
 acetyl-L-Phe-Gly
-
 Bos taurus
0.045
-
 acetyl-L-Phe-OCH2COOH
-
 Bos taurus
0.0598
-
 acetyl-L-Leu-OCH2COOH
-
 Bos taurus
0.522
-
 acetyl-DL-Phe-OCH2COOH
-
 Bos taurus
0.58
-
 acetyl-L-Phe-D-Phe-Gly
-
 Bos taurus
1.25
-
 acetyl-Gly-OCH2COOH
-
 Bos taurus
1.3
-
 acetyl-D-Phe-Gly
-
 Bos taurus
2.11
-
 acetyl-D-Leu-OCH2COOH
-
 Bos taurus
2.25
-
 acetyl-D-Phe-OCH2COOH
-
 Bos taurus