General Stability | Organism |
---|---|
repeated freeze-thaw cycles have no effect on enzyme activity | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ascorbate | above 0.4 mM for the purified enzyme, above 1.5 mM for the enzyme in crude extract | Rattus norvegicus | |
Cu2+ | at higher concentration | Rattus norvegicus | |
diethyldithiocarbamate | copper chelator | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km for D-Tyr-Val-Gly depends on concentration of ascorbate, Km for ascorbate is constant over a wide range of D-Tyr-Val-Gly concentration | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
secretory granule | - |
Rattus norvegicus | 30141 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | a copper protein | Rattus norvegicus | |
Cu2+ | Cu2+ is absolutely required for optimal activity | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptidylglycine + ascorbate + O2 | Rattus norvegicus | - |
peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partially | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pituitary gland | anterior, intermediate and posterior | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adrenocorticotrophic hormone(9-14) + ascorbate + O2 | - |
Rattus norvegicus | adrenocorticotrophic hormone(9-13)-2-hydroxyglycine + dehydroascorbate + H2O | - |
? | |
alpha-N-acetyl-adrenocorticotrophic hormone(1-14) + ascorbate + O2 | - |
Rattus norvegicus | alpha-N-acetyl-adrenocorticotrophic hormone(1-13)-2-hydroxyglycine + dehydroascorbate + H2O | - |
? | |
D-Tyr-Val-Gly + ascorbate + O2 | - |
Rattus norvegicus | D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O | - |
? | |
additional information | substrates are also physiologically relevant peptides related to alpha-melanotropine | Rattus norvegicus | ? | - |
? | |
peptidylglycine + ascorbate + O2 | - |
Rattus norvegicus | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
peptidylglycine + ascorbate + O2 | COOH-terminal glycine | Rattus norvegicus | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
incubation for 12-15 h, 47% loss of activity | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ascorbate | dependent on | Rattus norvegicus |