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Literature summary for 1.14.16.4 extracted from

  • Windahl, M.; Boesen, J.; Karlsen, P.; Christensen, H.
    Expression, purification and enzymatic characterization of the catalytic domains of human tryptophan hydroxylase isoforms (2009), Protein J., 28, 400-406.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
catalytic domain of TPH1 (DELTA1-99 TPH1 DELTA414-444) amplified, the cTPH/NdeI/XhoI gene cloned into the pET26 vector, expressed in Escherichia coli BL21(DE3) cells Homo sapiens
catalytic domain of TPH2 (DELTA1-145 TPH2 DELTA460-490) amplified, the cTPH/NdeI/XhoI gene cloned into the pET26 vector, expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
tryptophan substrate inhibition of the catalytic domain of TPH1 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
L-tryptophan catalytic domain of TPH2 Homo sapiens
0.0228
-
L-tryptophan catalytic domain of TPH1 Homo sapiens
0.0265
-
tetrahydrobiopterin catalytic domain of TPH2 Homo sapiens
0.109
-
O2 catalytic domain of TPH1 Homo sapiens
0.273
-
O2 catalytic domain of TPH2 Homo sapiens
0.315
-
tetrahydrobiopterin catalytic domain of TPH1 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36100
-
catalytic domain of TPH1, gel filtration Homo sapiens
36200
-
catalytic domain of TPH2, gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P17752
-
-
Homo sapiens Q8IWU9
-
-

Purification (Commentary)

Purification (Comment) Organism
by gel filtration and ultrafiltration Homo sapiens

Storage Stability

Storage Stability Organism
-80°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tryptophan + tetrahydrobiopterin + O2
-
Homo sapiens 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin
-
?

Synonyms

Synonyms Comment Organism
TPH1
-
Homo sapiens
TPH2
-
Homo sapiens
tryptophan hydroxylase isoform 1
-
Homo sapiens
tryptophan hydroxylase isoform 2
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.383
-
O2 catalytic domain of TPH1 Homo sapiens
2.167
-
tetrahydrobiopterin catalytic domain of TPH1 Homo sapiens
2.67
-
L-tryptophan catalytic domain of TPH1 Homo sapiens
4.567
-
tetrahydrobiopterin catalytic domain of TPH2 Homo sapiens
5.03
-
L-tryptophan catalytic domain of TPH2 Homo sapiens
6.73
-
O2 catalytic domain of TPH2 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
tetrahydrobiopterin
-
Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.072
-
L-tryptophan catalytic domain of TPH1 Homo sapiens