Cloned (Comment) | Organism |
---|---|
catalytic domain of TPH1 (DELTA1-99 TPH1 DELTA414-444) amplified, the cTPH/NdeI/XhoI gene cloned into the pET26 vector, expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
catalytic domain of TPH2 (DELTA1-145 TPH2 DELTA460-490) amplified, the cTPH/NdeI/XhoI gene cloned into the pET26 vector, expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tryptophan | substrate inhibition of the catalytic domain of TPH1 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
L-tryptophan | catalytic domain of TPH2 | Homo sapiens | |
0.0228 | - |
L-tryptophan | catalytic domain of TPH1 | Homo sapiens | |
0.0265 | - |
tetrahydrobiopterin | catalytic domain of TPH2 | Homo sapiens | |
0.109 | - |
O2 | catalytic domain of TPH1 | Homo sapiens | |
0.273 | - |
O2 | catalytic domain of TPH2 | Homo sapiens | |
0.315 | - |
tetrahydrobiopterin | catalytic domain of TPH1 | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36100 | - |
catalytic domain of TPH1, gel filtration | Homo sapiens |
36200 | - |
catalytic domain of TPH2, gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P17752 | - |
- |
Homo sapiens | Q8IWU9 | - |
- |
Purification (Comment) | Organism |
---|---|
by gel filtration and ultrafiltration | Homo sapiens |
Storage Stability | Organism |
---|---|
-80°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + tetrahydrobiopterin + O2 | - |
Homo sapiens | 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TPH1 | - |
Homo sapiens |
TPH2 | - |
Homo sapiens |
tryptophan hydroxylase isoform 1 | - |
Homo sapiens |
tryptophan hydroxylase isoform 2 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.383 | - |
O2 | catalytic domain of TPH1 | Homo sapiens | |
2.167 | - |
tetrahydrobiopterin | catalytic domain of TPH1 | Homo sapiens | |
2.67 | - |
L-tryptophan | catalytic domain of TPH1 | Homo sapiens | |
4.567 | - |
tetrahydrobiopterin | catalytic domain of TPH2 | Homo sapiens | |
5.03 | - |
L-tryptophan | catalytic domain of TPH2 | Homo sapiens | |
6.73 | - |
O2 | catalytic domain of TPH2 | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
tetrahydrobiopterin | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.072 | - |
L-tryptophan | catalytic domain of TPH1 | Homo sapiens |