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Literature summary for 1.14.16.4 extracted from

  • Nielsen, M.S.; Petersen, C.R.; Munch, A.; Vendelboe, T.V.; Boesen, J.; Harris, P.; Christensen, H.E.
    A simple two step procedure for purification of the catalytic domain of chicken tryptophan hydroxylase 1 in a form suitable for crystallization (2008), Protein Expr. Purif., 57, 116-126.
    View publication on PubMed
Search for more literature for 1.14.16.4

Activating Compound

Activating Compound Comment Organism Structure
DTT
-
 Gallus gallus

Cloned(Commentary)

Cloned (Comment) Organism
high yield expression of the catalytic domain of chicken TPH isoform 1, comprising residues 1-100 and 415-445, fused to ubiquitin in Escherichia coli strain BL21(DE3) Gallus gallus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified enzyme without 7,8-dihydro-L-biopterin, sitting drop vapour diffusion method, 0.002 ml of 4.2 mg/ml protein in 20 mM Tris/NaOH, 100 mM (NH4)2SO4 pH 8.5, is mixed with 0.002 ml of reservoir solution containing 0.2 M imidazole malate, pH 8.5, 22.5% PEG 10000, and tetrahydrobiopterin in excess, X-ray diffraction structure determination and analysis at 3.0 A reslution Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
L-tryptophan substrate inhibition at concentrations above 0.015 mM Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0077
-
 L-tryptophan pH 7.0, 15°C, recombinant TPH1 Gallus gallus
0.039
-
 O2 pH 7.0, 15°C, recombinant TPH1 Gallus gallus
0.324
-
 tetrahydrobiopterin pH 7.0, 15°C, recombinant TPH1 Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tryptophan + tetrahydrobiopterin + O2 Gallus gallus first and rate-determining step in the biosynthesis of the neurotransmitter serotonin 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus P70080 isozyme TPH1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant catalytic domain of chicken TPH isoform 1 from Escherichia coli strain BL21(DE3) by anion exchange chromatography and gel filtration to homogeneity, release from the fusion by in vivo cleavage by a co-expressed ubiquitin-specific, carboxy-terminal protease Gallus gallus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.6
-
 purified recombinant TPH1 Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tryptophan + tetrahydrobiopterin + O2
-
 Gallus gallus 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin
-
 ?
L-tryptophan + tetrahydrobiopterin + O2 first and rate-determining step in the biosynthesis of the neurotransmitter serotonin Gallus gallus 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin
-
 ?

Synonyms

Synonyms Comment Organism
TPH1
-
 Gallus gallus
tryptophan hydroxylase 1
-
 Gallus gallus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
15
-
 assay at Gallus gallus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
22.8
-
 O2 pH 7.0, 15°C, recombinant TPH1 Gallus gallus
32
-
 L-tryptophan pH 7.0, 15°C, recombinant TPH1 Gallus gallus
43
-
 tetrahydrobiopterin pH 7.0, 15°C, recombinant TPH1 Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Gallus gallus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.164
-
 L-tryptophan pH 7.0, 15°C, recombinant TPH1 Gallus gallus