Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.16.4 extracted from

  • Murphy, K.L.; Zhang, X.; Gainetdinov, R.R.; Beaulieu, J.M.; Caron, M.G.
    A regulatory domain in the N terminus of tryptophan hydroxylase 2 controls enzyme expression (2008), J. Biol. Chem., 283, 13216-13224.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
genes TPH1 and TPH2, expression analysis, overview, expression of wild-type TPH1 and TPH2 in rat PC-12 cells, in vitro transcription and translation using reticulocyte lysate, the TPH2 extended N-terminal domain plays a role in modulating the enzyme expression, expression of mutant HA-TPH2-(1-41)-TPH1 in HEK-293 amd PC-12 cells, the expression level of the chimeric protein is significantly decreased compared with TPH1 in both cell lines and is more similar to that of TPH2, the N-terminal regulatory determinant is transferable, overview Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information construction of a chimeric protein HA-TPH2-(1-41)-TPH1 composed of the first 41 amino acids of TPH2 appended to the N terminus of TPH1, the expression level of the chimeric protein is significantly decreased compared with TPH1 in HEK-293 and PC-12 cell lines and is more similar to that of TPH2 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tryptophan + tetrahydrobiopterin + O2 Homo sapiens differences in the regulation of isozyme TPH2 versus isozyme TPH1, overview 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8IWU9
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tryptophan + tetrahydrobiopterin + O2
-
Homo sapiens 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin
-
?
L-tryptophan + tetrahydrobiopterin + O2 differences in the regulation of isozyme TPH2 versus isozyme TPH1, overview Homo sapiens 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin
-
?

Synonyms

Synonyms Comment Organism
TPH2
-
Homo sapiens
tryptophan hydroxylase 2
-
Homo sapiens