Protein Variants | Comment | Organism |
---|---|---|
additional information | polymorphism rs11178997 of the TPH2 promoter significantly reduces TPH2 transcriptional activity by 22% in primary serotonergic neurons. In contrast, no significant differences in promoter activity are observed for the G- and T-alleles of rs4570625. Reduced binding of the transcription factor POU3F2, i.e. Brn-2 or N-Oct-3, to the A-allele occur with the polymorphism rs11178997, while overexpression of POU3F2 results in a robust activation of the TPH2 promoter by about 2.7fold. The human TPH2 promoter is active in rat serotonergic raphe neurons | Rattus norvegicus |
additional information | polymorphism rs11178997 of the TPH2 promoter significantly reduces TPH2 transcriptional activity by 7% in SHP-77 cells. In contrast, no significant differences in promoter activity are observed for the G- and T-alleles of rs4570625. Reduced binding of the transcription factor POU3F2, i.e. Brn-2 or N-Oct-3, to the A-allele occur with the polymorphism rs11178997, while overexpression of POU3F2 results in a robust activation of the TPH2 promoter by about 2.7fold, functional assays of TPH2 regulatory region variants, overview | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + tetrahydrobiopterin + O2 | Homo sapiens | TPH2 is the rate-limiting enzyme in brain serotonin/5-hydroxytryptamine biosynthesis | 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin | - |
? | |
L-tryptophan + tetrahydrobiopterin + O2 | Rattus norvegicus | TPH2 is the rate-limiting enzyme in brain serotonin/5-hydroxytryptamine biosynthesis | 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozymes TPH1 and TPH2 | - |
Rattus norvegicus | Q8CGU9 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
brain | raphe nuclei | Rattus norvegicus | - |
neuron | primary serotonergic, from raphe nuclei | Rattus norvegicus | - |
SHP-77 cell | human small cell lung carcinoma cell line, SHP-77 cells preferentially express TPH1 over TPH2 mRNA | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + tetrahydrobiopterin + O2 | - |
Homo sapiens | 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin | - |
? | |
L-tryptophan + tetrahydrobiopterin + O2 | - |
Rattus norvegicus | 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin | - |
? | |
L-tryptophan + tetrahydrobiopterin + O2 | TPH2 is the rate-limiting enzyme in brain serotonin/5-hydroxytryptamine biosynthesis | Homo sapiens | 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin | - |
? | |
L-tryptophan + tetrahydrobiopterin + O2 | TPH2 is the rate-limiting enzyme in brain serotonin/5-hydroxytryptamine biosynthesis | Rattus norvegicus | 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TPH1 | - |
Homo sapiens |
TPH2 | - |
Homo sapiens |
TPH2 | - |
Rattus norvegicus |
tryptophan hydroxylase 2 | - |
Homo sapiens |
tryptophan hydroxylase 2 | - |
Rattus norvegicus |