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Literature summary for 1.14.16.4 extracted from

  • Moran, G.R.; Phillips, R.S.; Fitzpatrick, P.F.
    Influence of steric bulk and electrostatics on the hHydroxylation regiospecificity of tryptophan hydroxylase: characterization of methyltryptophans and azatryptophans as substrates (1999), Biochemistry, 38, 16283-16289.
    View publication on PubMed
Search for more literature for 1.14.16.4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0021
-
 L-tryptophan
-
 Oryctolagus cuniculus
0.0151
-
 6-methyltryptophan
-
 Oryctolagus cuniculus
0.0375
-
 7-methyltryptophan
-
 Oryctolagus cuniculus
0.0662
-
 7-azatryptophan
-
 Oryctolagus cuniculus
0.0865
-
 2-azaisotryptophan
-
 Oryctolagus cuniculus
0.262
-
 4-azatryptophan
-
 Oryctolagus cuniculus
0.295
-
 6-azatryptophan
-
 Oryctolagus cuniculus
0.994
-
 4-methyltryptophan
-
 Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N- and C-terminal truncated mutant enzyme Oryctolagus cuniculus

Reaction

Reaction Comment Organism Reaction ID
L-tryptophan + a 5,6,7,8-tetrahydropteridine + O2 = 5-hydroxy-L-tryptophan + a 4a-hydroxy-5,6,7,8-tetrahydropteridine hydroxylation regiospecificity Oryctolagus cuniculus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-azaisotryptophan + 6-methyltetrahydropterin + O2 recombinant N- and C-terminal truncated enzyme Oryctolagus cuniculus 5-hydroxy-2-azaisotryptophan + 6-hydroxy-2-azaisotryptophan + 6-methyldihydropterin + H2O
-
 ?
4-azatryptophan + 6-methyltetrahydropterin + O2 recombinant N- and C-terminal truncated enzyme Oryctolagus cuniculus 5-hydroxy-4-azatryptophan + 6-methyldihydropterin + H2O
-
 ?
4-methyltryptophan + 6-methyltetrahydropterin + O2 recombinant N- and C-terminal truncated enzyme Oryctolagus cuniculus 5-hydroxy-4-methyltryptophan + 6-methyldihydropterin + H2O
-
 ?
5-methyltryptophan + 6-methyltetrahydropterin + O2 recombinant N- and C-terminal truncated enzyme Oryctolagus cuniculus 5-hydroxymethyltryptophan + 5-hydroxy-4-methyltryptophan + 6-methyldihydropterin + H2O more than 99% 5-hydroxymethyltryptophan ?
6-azatryptophan + 6-methyltetrahydropterin + O2 recombinant N- and C-terminal truncated enzyme Oryctolagus cuniculus 5-hydroxy-6-azatryptophan + 6-methyldihydropterin + H2O
-
 ?
6-methyltryptophan + 6-methyltetrahydropterin + O2 recombinant N- and C-terminal truncated enzyme Oryctolagus cuniculus 5-hydroxy-6-methyltryptophan + 6-methyldihydropterin + H2O
-
 ?
7-azatryptophan + 6-methyltetrahydropterin + O2 recombinant N- and C-terminal truncated enzyme Oryctolagus cuniculus 5-hydroxy-7-azatryptophan + 6-methyldihydropterin + H2O
-
 ?
7-methyltryptophan + 6-methyltetrahydropterin + O2 recombinant N- and C-terminal truncated enzyme Oryctolagus cuniculus 5-hydroxy-7-methyltryptophan + 6-methyldihydropterin + H2O
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0733
-
 6-methyltryptophan
-
 Oryctolagus cuniculus
0.207
-
 L-tryptophan
-
 Oryctolagus cuniculus
0.217
-
 2-azaisotryptophan
-
 Oryctolagus cuniculus
0.252
-
 7-azatryptophan
-
 Oryctolagus cuniculus
0.273
-
 4-methyltryptophan
-
 Oryctolagus cuniculus
0.315
-
 7-methyltryptophan
-
 Oryctolagus cuniculus
0.358
-
 4-azatryptophan
-
 Oryctolagus cuniculus
0.533
-
 6-azatryptophan
-
 Oryctolagus cuniculus