KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0021 | - |
L-tryptophan | - |
Oryctolagus cuniculus | |
0.0151 | - |
6-methyltryptophan | - |
Oryctolagus cuniculus | |
0.0375 | - |
7-methyltryptophan | - |
Oryctolagus cuniculus | |
0.0662 | - |
7-azatryptophan | - |
Oryctolagus cuniculus | |
0.0865 | - |
2-azaisotryptophan | - |
Oryctolagus cuniculus | |
0.262 | - |
4-azatryptophan | - |
Oryctolagus cuniculus | |
0.295 | - |
6-azatryptophan | - |
Oryctolagus cuniculus | |
0.994 | - |
4-methyltryptophan | - |
Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant N- and C-terminal truncated mutant enzyme | Oryctolagus cuniculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-tryptophan + a 5,6,7,8-tetrahydropteridine + O2 = 5-hydroxy-L-tryptophan + a 4a-hydroxy-5,6,7,8-tetrahydropteridine | hydroxylation regiospecificity | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-azaisotryptophan + 6-methyltetrahydropterin + O2 | recombinant N- and C-terminal truncated enzyme | Oryctolagus cuniculus | 5-hydroxy-2-azaisotryptophan + 6-hydroxy-2-azaisotryptophan + 6-methyldihydropterin + H2O | - |
? | |
4-azatryptophan + 6-methyltetrahydropterin + O2 | recombinant N- and C-terminal truncated enzyme | Oryctolagus cuniculus | 5-hydroxy-4-azatryptophan + 6-methyldihydropterin + H2O | - |
? | |
4-methyltryptophan + 6-methyltetrahydropterin + O2 | recombinant N- and C-terminal truncated enzyme | Oryctolagus cuniculus | 5-hydroxy-4-methyltryptophan + 6-methyldihydropterin + H2O | - |
? | |
5-methyltryptophan + 6-methyltetrahydropterin + O2 | recombinant N- and C-terminal truncated enzyme | Oryctolagus cuniculus | 5-hydroxymethyltryptophan + 5-hydroxy-4-methyltryptophan + 6-methyldihydropterin + H2O | more than 99% 5-hydroxymethyltryptophan | ? | |
6-azatryptophan + 6-methyltetrahydropterin + O2 | recombinant N- and C-terminal truncated enzyme | Oryctolagus cuniculus | 5-hydroxy-6-azatryptophan + 6-methyldihydropterin + H2O | - |
? | |
6-methyltryptophan + 6-methyltetrahydropterin + O2 | recombinant N- and C-terminal truncated enzyme | Oryctolagus cuniculus | 5-hydroxy-6-methyltryptophan + 6-methyldihydropterin + H2O | - |
? | |
7-azatryptophan + 6-methyltetrahydropterin + O2 | recombinant N- and C-terminal truncated enzyme | Oryctolagus cuniculus | 5-hydroxy-7-azatryptophan + 6-methyldihydropterin + H2O | - |
? | |
7-methyltryptophan + 6-methyltetrahydropterin + O2 | recombinant N- and C-terminal truncated enzyme | Oryctolagus cuniculus | 5-hydroxy-7-methyltryptophan + 6-methyldihydropterin + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0733 | - |
6-methyltryptophan | - |
Oryctolagus cuniculus | |
0.207 | - |
L-tryptophan | - |
Oryctolagus cuniculus | |
0.217 | - |
2-azaisotryptophan | - |
Oryctolagus cuniculus | |
0.252 | - |
7-azatryptophan | - |
Oryctolagus cuniculus | |
0.273 | - |
4-methyltryptophan | - |
Oryctolagus cuniculus | |
0.315 | - |
7-methyltryptophan | - |
Oryctolagus cuniculus | |
0.358 | - |
4-azatryptophan | - |
Oryctolagus cuniculus | |
0.533 | - |
6-azatryptophan | - |
Oryctolagus cuniculus |