Cloned (Comment) | Organism |
---|---|
expression of wild-type and N- and C-terminal truncated mutant enzyme in Escherichia coli | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0058 | - |
L-tryptophan | recombinant N- and C-terminal truncated enzyme, cofactor 6-methyltetrahydrobipterin | Oryctolagus cuniculus | |
0.0142 | - |
tetrahydrobiopterin | recombinant N- and C-terminal truncated enzyme, substrate phenylalanine | Oryctolagus cuniculus | |
0.0146 | - |
6-methyltetrahydropterin | recombinant N- and C-terminal truncated enzyme, substrate phenylalanine | Oryctolagus cuniculus | |
0.0479 | - |
L-tryptophan | recombinant N- and C-terminal truncated enzyme, cofactor tetrahydrobiopterin | Oryctolagus cuniculus | |
0.0606 | - |
L-phenylalanine | recombinant N- and C-terminal truncated enzyme, cofactor tetrahydrobiopterin | Oryctolagus cuniculus | |
0.102 | - |
L-phenylalanine | recombinant N- and C-terminal truncated enzyme, cofactor 6-methyltetrahydropterin | Oryctolagus cuniculus | |
0.135 | - |
tetrahydrobiopterin | recombinant N- and C-terminal truncated enzyme, substrate tryptophan | Oryctolagus cuniculus | |
0.177 | - |
6-methyltetrahydropterin | recombinant N- and C-terminal truncated enzyme, substrate tryptophan | Oryctolagus cuniculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | N- and C-terminal recombinant enzyme, iron is required for activity | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant N- and C-terminal truncated mutant enzyme | Oryctolagus cuniculus |
Storage Stability | Organism |
---|---|
N- and C-terminal truncated recombinant enzyme, -70°C, 100 mM ammonium sulfate, ferrous iron, 10% glycerol, 2 mM dithiothreitiol, pH 7.0, indefinitely, no loss of activity | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + tetrahydropteridine + O2 | - |
Oryctolagus cuniculus | tyrosine + dihydropteridine + H2O | - |
? | |
L-tryptophan + tetrahydrobiopterin + O2 | - |
Oryctolagus cuniculus | 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin | - |
? | |
L-tryptophan + tetrahydropteridine + O2 | - |
Oryctolagus cuniculus | 5-hydroxy-L-tryptophan + dihydropteridine + H2O | - |
? |