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Literature summary for 1.14.16.4 extracted from

  • Nakata, H.; Fujisawa, H.
    Simple and rapid purification of tryptophan 5-monooxygenase from rabbit brain by affinity chromatography (1981), J. Biochem., 90, 567-569.
    View publication on PubMed
Search for more literature for 1.14.16.4

General Stability

General Stability Organism
EDTA stabilizes Oryctolagus cuniculus
glycerol stabilizes Oryctolagus cuniculus
Tween 20 stabilizes Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
brain enzyme, calcium phosphate gel, dimethyltetrahydropteridine-agarose Rattus norvegicus
brain enzyme, calcium phosphate gel, dimethyltetrahydropteridine-agarose Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-
brain
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.015
-
-
 Oryctolagus cuniculus
0.0271
-
 brain tryptophan hydroxylase Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tryptophan + tetrahydropteridine + O2
-
 Rattus norvegicus 5-hydroxy-L-tryptophan + dihydropteridine + H2O
-
 ?
L-tryptophan + tetrahydropteridine + O2
-
 Oryctolagus cuniculus 5-hydroxy-L-tryptophan + dihydropteridine + H2O
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 complete loss of activity in absence of stabilizing agents after 24 h Oryctolagus cuniculus