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Literature summary for 1.14.16.2 extracted from
- Glu434 is an important amino acid residue for the activity, structure and stability of tyrosine hydroxylase of the silkworm, Bombyx mori (2013), Process Biochem., 48, 1665-1673.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Bombyx mori |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E434A | the mutant shows 35.6% of wild type activity. Furthermore, the mutation dramatically reduces its substrate affinity for tetrahydrobiopterin and decreases its activation by Fe2+ | Bombyx mori |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| guanidine hydrochloride | no activity at guanidine hydrochloride concentrations exceeding 0.6 M | Bombyx mori |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0263 | - |
L-tyrosine | recombinant wild type enzyme, at pH 7.5 and 37°C | Bombyx mori | |
| 0.029 | - |
L-tyrosine | native wild type enzyme, at pH 7.5 and 37°C | Bombyx mori | |
| 0.0428 | - |
L-tyrosine | mutant enzyme E434A, at pH 7.5 and 37°C | Bombyx mori | |
| 0.0471 | - |
tetrahydrobiopterin | native wild type enzyme, at pH 7.5 and 37°C | Bombyx mori | |
| 0.0519 | - |
tetrahydrobiopterin | recombinant wild type enzyme, at pH 7.5 and 37°C | Bombyx mori | |
| 0.341 | - |
tetrahydrobiopterin | mutant enzyme E434A, at pH 7.5 and 37°C | Bombyx mori | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | activates | Bombyx mori | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66200 | - |
x * 66200, SDS-PAGE | Bombyx mori |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-tyrosine + tetrahydrobiopterin + O2 | Bombyx mori | - |
3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bombyx mori | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| heparin-Sepharose column chromatography, DEAE-Sepharose column chromatography, and Sephacryl S-200HR gel filtration | Bombyx mori |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bombyx mori | - |
| fat body | - |
Bombyx mori | - |
| larva | - |
Bombyx mori | - |
| nerve cord | - |
Bombyx mori | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-tyrosine + tetrahydrobiopterin + O2 | - |
Bombyx mori | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 66200, SDS-PAGE | Bombyx mori |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| tyrosine hydroxylase | - |
Bombyx mori |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 65 | minimal aggregation occurs for wild type enzyme after heating at 40°C-45 for 60 min. A steep decrease in activity is observed between 40 and 65°C, and a complete loss of activity occurs at temperatures above 70°C | Bombyx mori |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| tetrahydrobiopterin | - |
Bombyx mori | |
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