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Literature summary for 1.14.16.2 extracted from

  • Briggs, G.D.; Gordon, S.L.; Dickson, P.W.
    Mutational analysis of catecholamine binding in tyrosine hydroxylase (2011), Biochemistry, 50, 1545-1555.
    View publication on PubMed
Search for more literature for 1.14.16.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Homo sapiens

Protein Variants

Protein Variants Comment Organism
A297L outer edge of the active site Homo sapiens
E332D active site residue, 10fold reduction in activity, close to the catalytic iron Homo sapiens
E332Q active site residue, no activity, close to the catalytic iron Homo sapiens
F300A active site residue Homo sapiens
F300Y active site residue Homo sapiens
L294A protrudes into catalytic cleft Homo sapiens
L294Y protrudes into catalytic cleft Homo sapiens
Y371F active site residue, close to the catalytic iron Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
dopamine
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0056
-
 tetrahydrobiopterin wild type, phosphorylated protein, pH 7.2, 30°C Homo sapiens
0.0068
-
 tetrahydrobiopterin wild type protein, dopamine bound but removed from low-affinity site, pH 7.2, 30°C Homo sapiens
0.0072
-
 tetrahydrobiopterin E332D phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
0.0083
-
 tetrahydrobiopterin E332D mutant protein, pH 7.2, 30°C Homo sapiens
0.0099
-
 tetrahydrobiopterin L294Y phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
0.012
-
 tetrahydrobiopterin F300Y phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
0.014
-
 tetrahydrobiopterin L294A mutant protein, pH 7.2, 30°C Homo sapiens
0.014
-
 tetrahydrobiopterin wild type protein, pH 7.2, 30°C Homo sapiens
0.015
-
 tetrahydrobiopterin L294Y mutant protein, pH 7.2, 30°C Homo sapiens
0.017
-
 tetrahydrobiopterin A297L phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
0.017
-
 tetrahydrobiopterin Y371F mutant protein, pH 7.2, 30°C Homo sapiens
0.02
-
 tetrahydrobiopterin A297L mutant protein, pH 7.2, 30°C Homo sapiens
0.026
-
 tetrahydrobiopterin F300Y mutant protein, pH 7.2, 30°C Homo sapiens
0.027
-
 tetrahydrobiopterin A297L mutant protein, dopamine bound to the high-affinity site, pH 7.2, 30°C Homo sapiens
0.027
-
 tetrahydrobiopterin Y371F mutant protein, dopamine bound to the high-affinity site, pH 7.2, 30°C Homo sapiens
0.029
-
 tetrahydrobiopterin L294A phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
0.029
-
 tetrahydrobiopterin Y371F phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
0.034
-
 tetrahydrobiopterin E332D mutant protein, dopamine bound to the high-affinity site, pH 7.2, 30°C Homo sapiens
0.054
-
 tetrahydrobiopterin F300A mutant protein, pH 7.2, 30°C Homo sapiens
0.07
-
 tetrahydrobiopterin F300A phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
0.134
-
 tetrahydrobiopterin wild type protein, dopamine bound to the high-affinity site, pH 7.2, 30°C Homo sapiens
0.356
-
 tetrahydrobiopterin wild type protein, in the presence of dopamine, pH 7.2, 30°C Homo sapiens
0.507
-
 tetrahydrobiopterin wild type, phosphorylated protein, in the presence of dopamine, pH 7.2, 30°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P07101
-
-

Purification (Commentary)

Purification (Comment) Organism
heparin Sepharose chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tyrosine + tetrahydrobiopterin + O2
-
 Homo sapiens 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin + H2O
-
 ?

Synonyms

Synonyms Comment Organism
tyrosine hydroxylase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.017
-
 tetrahydrobiopterin wild type protein, in the presence of 0.01 mM dopamine, pH 7.2, 30°C Homo sapiens
0.047
-
 tetrahydrobiopterin E332D phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
0.06
-
 tetrahydrobiopterin E332D mutant protein, pH 7.2, 30°C Homo sapiens
0.068
-
 tetrahydrobiopterin L294Y phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
0.088
-
 tetrahydrobiopterin F300A mutant protein, pH 7.2, 30°C Homo sapiens
0.11
-
 tetrahydrobiopterin F300A phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
0.12
-
 tetrahydrobiopterin L294Y mutant protein, pH 7.2, 30°C Homo sapiens
0.14
-
 tetrahydrobiopterin L294A phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
0.18
-
 tetrahydrobiopterin L294A mutant protein, pH 7.2, 30°C Homo sapiens
0.2
-
 tetrahydrobiopterin Y371F mutant protein, pH 7.2, 30°C Homo sapiens
0.22
-
 tetrahydrobiopterin A297L phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
0.27
-
 tetrahydrobiopterin A297L mutant protein, pH 7.2, 30°C Homo sapiens
0.27
-
 tetrahydrobiopterin F300Y phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
0.3
-
 tetrahydrobiopterin F300Y mutant protein, pH 7.2, 30°C Homo sapiens
0.3
-
 tetrahydrobiopterin Y371F phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
0.47
-
 tetrahydrobiopterin wild type, phosphorylated protein, pH 7.2, 30°C Homo sapiens
0.58
-
 tetrahydrobiopterin wild type protein, pH 7.2, 30°C Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000256
-
 wild type, phosphorylated protein, pH 7.2, 30°C Homo sapiens dopamine
0.0004
-
 wild type protein, pH 7.2, 30°C Homo sapiens dopamine
0.00093
-
 F300Y mutant protein, pH 7.2, 30°C Homo sapiens dopamine
0.00098
-
 Y371F mutant protein, pH 7.2, 30°C Homo sapiens dopamine
0.00107
-
 L294A mutant protein, pH 7.2, 30°C Homo sapiens dopamine
0.00143
-
 E332D mutant protein, pH 7.2, 30°C Homo sapiens dopamine
0.00248
-
 E332D phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens dopamine
0.0136
-
 E332D phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens dopamine

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.7
-
 tetrahydrobiopterin F300A mutant protein, pH 7.2, 30°C Homo sapiens
1.7
-
 tetrahydrobiopterin F300A phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
5
-
 tetrahydrobiopterin L294A phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
6.5
-
 tetrahydrobiopterin E332D phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
7
-
 tetrahydrobiopterin L294Y phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
7.3
-
 tetrahydrobiopterin E332D mutant protein, pH 7.2, 30°C Homo sapiens
7.7
-
 tetrahydrobiopterin L294Y mutant protein, pH 7.2, 30°C Homo sapiens
10.7
-
 tetrahydrobiopterin Y371F phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
11.2
-
 tetrahydrobiopterin F300Y mutant protein, pH 7.2, 30°C Homo sapiens
11.7
-
 tetrahydrobiopterin Y371F mutant protein, pH 7.2, 30°C Homo sapiens
12.5
-
 tetrahydrobiopterin A297L phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
13
-
 tetrahydrobiopterin L294A mutant protein, pH 7.2, 30°C Homo sapiens
13.7
-
 tetrahydrobiopterin A297L mutant protein, pH 7.2, 30°C Homo sapiens
21.8
-
 tetrahydrobiopterin F300Y phosphorylated mutant protein, pH 7.2, 30°C Homo sapiens
45.7
-
 tetrahydrobiopterin wild type protein, pH 7.2, 30°C Homo sapiens
89.3
-
 tetrahydrobiopterin wild type, phosphorylated protein, pH 7.2, 30°C Homo sapiens