Cloned (Comment) | Organism |
---|---|
4 isoforms, overexpression in Escherichia coli, phosphorylation-free | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tyrosine | substrate inhibition, with (6R)-L-erythro-tetrahydrobiopterin | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km dependent on cosubstrate type and concentration, overview for 4 isoforms | Homo sapiens | |
0.055 | - |
tyrosine | recombinant isoform hTH2, with 6-methyltetrahydropterin | Homo sapiens | |
0.066 | - |
tyrosine | recombinant isoform hTH4, with 6-methyltetrahydropterin | Homo sapiens | |
0.074 | - |
tyrosine | recombinant isoform hTH3, with 6-methyltetrahydropterin | Homo sapiens | |
0.166 | - |
tyrosine | recombinant isoform hTH1, with 6-methyltetrahydropterin | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | required | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
4 isoforms, gel filtration | Homo sapiens |
60000 | - |
4 * 60000, 4 isoforms, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + (6R)-L-erythro-tetrahydrobiopterin + O2 | Homo sapiens | probable regulatory role of cosubstrate for all 4 isoforms | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
4 isoforms hTH1, hTH2, hTH3, hTH4 | - |
Purification (Comment) | Organism |
---|---|
4 isoforms, recombinant from Escherichia coli | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + (6R)-L-erythro-tetrahydrobiopterin + O2 | cosubstrate has a regulatory role for all 4 isoforms | Homo sapiens | ? | - |
? | |
L-tyrosine + (6R)-L-erythro-tetrahydrobiopterin + O2 | probable regulatory role of cosubstrate for all 4 isoforms | Homo sapiens | ? | - |
? | |
L-tyrosine + (6RS)-L-erythro-tetrahydrobiopterin + O2 | - |
Homo sapiens | ? | - |
? | |
L-tyrosine + (6S)-L-erythro-tetrahydrobiopterin + O2 | - |
Homo sapiens | ? | - |
? | |
L-tyrosine + 6-methyltetrahydropterin + O2 | - |
Homo sapiens | ? | - |
? | |
L-tyrosine + tetrahydrobiopterin + O2 | - |
Homo sapiens | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | 3,4-dihydroxy-L-phenylalanine is identical with dopa | ? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 60000, 4 isoforms, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
TH | - |
Homo sapiens |
tyrosine-3-mono-oxygenase | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | 4 isoforms | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | 4 isoforms | Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
preincubation at 37°C for 2 h below, enzyme isoforms are unstable, especially isoforms hTH1 and hTH3 | Homo sapiens |
7 | - |
preincubation at 37°C for 2 h above, isoforms are stable | Homo sapiens |
8 | - |
isoforms, optimal stability at | Homo sapiens |