Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.16.2 extracted from

  • Hillas, P.J.; Fitzpatrick, P.F.
    A mechanism for hydroxylation by tyrosine hydroxylase based on partitioning of substituted phenylalanines (1996), Biochemistry, 35, 6969-6975.
    View publication on PubMed
Search for more literature for 1.14.16.2

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
L-tyrosine + a 5,6,7,8-tetrahydropteridine + O2 = L-dopa + a 4a-hydroxy-5,6,7,8-tetrahydropteridine mechanism Rattus norvegicus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-bromo-L-phenylalanine + 6-methyltetrahydropterin + O2 low activity Rattus norvegicus ?
-
 ?
4-chloro-L-phenylalanine + 6-methyltetrahydropterin + O2
-
 Rattus norvegicus ?
-
 ?
4-fluoro-L-phenylalanine + 6-methyltetrahydropterin + O2
-
 Rattus norvegicus ?
-
 ?
4-methoxy-L-phenylalanine + 6-methyltetrahydropteridine + O2
-
 Rattus norvegicus ?
-
 ?
4-methyl-L-phenylalanine + 6-methyltetrahydropterin + O2
-
 Rattus norvegicus ?
-
 ?
L-phenylalanine + tetrahydropteridine + 2 O2
-
 Rattus norvegicus 3,4-dihydroxy-L-phenylalanine + dihydropteridine + 2 H2O
-
 ?
L-tyrosine + 6-methyltetrahydropterin + O2
-
 Rattus norvegicus ?
-
 ?
L-tyrosine + tetrahydrobiopterin + O2
-
 Rattus norvegicus 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O 3,4-dihydroxy-L-phenylalanine is identical with dopa ?
additional information 4-substituted substrate analogues get hydroxylated at position 4 or 3, the latter is preferred with big-sized substituents, multiply hydroxylated products occur as well Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
 assay at Rattus norvegicus