Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.16.1 extracted from

  • Antypa, A.; Rebello, C.; Biernacka, A.; Krajewski, K.; Cassam, J.; Mitchell, S.C.; Steventon, G.B.
    Post-translational activation of human phenylalanine 4-monooxygenase from an endobiotic to a xenobiotic enzyme by reactive oxygen and reactive nitrogen species (2010), Xenobiotica, 40, 319-330.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a recombinant protein Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
L-phenylalanine post-translational activation of human cDNA-expressed PAH, activator: NO (nitric oxide) (0.01 mM), Vmax: 33.05 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
0.06
-
L-phenylalanine post-translational activation of human cDNA-expressed PAH, activator: ONOO- (peroxynitrite)(1 mM), Vmax: 32.65 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
0.07
-
L-phenylalanine post-translational activation of human cDNA-expressed PAH, activator: HO (hydroxyl radical) (0.01 mM), Vmax: 32.3 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
0.12
-
L-phenylalanine post-translational activation of human cDNA-expressed PAH, activator: lysophosphatidylcholine (5 mM), Vmax: 3.72 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
0.12
-
L-phenylalanine post-translational activation of human cDNA-expressed PAH, activator: N-ethylmaleimide (50 mM), Vmax: 9.3 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
0.14
-
L-phenylalanine post-translational activation of human cDNA-expressed PAH, activator: H2O2 (0.1 mM), Vmax: 28.4 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
0.14
-
L-phenylalanine post-translational activation of human cDNA-expressed PAH, activator: L-Phe (4 mM), Vmax: 1.86 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
0.87
-
L-phenylalanine post-translational activation of PAH in human hepatic cytosol fractions, activator: NO (nitric oxide) (0.01 mM), Vmax: 274.5 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
1.22
-
L-phenylalanine post-translational activation of PAH in human hepatic cytosol fractions, activator: N-ethylmaleimide (50 mM), Vmax: 224 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
1.25
-
L-phenylalanine post-translational activation of PAH in human hepatic cytosol fractions, activator: ONOO- (peroxynitrite)(1 mM), Vmax: 260.4 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
1.5 2 L-phenylalanine post-translational activation of PAH in human hepatic cytosol fractions, activator: HO (hydroxyl radical) (0.01 mM), Vmax: 241.6 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
2.38
-
S-carboxymethyl-L-cysteine post-translational activation of human cDNA-expressed PAH, activator: NO (nitric oxide) (0.01 mM), Vmax: 42.32 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
2.75
-
S-carboxymethyl-L-cysteine post-translational activation of human cDNA-expressed PAH, activator: H2O2 (0.1 mM), Vmax: 36.5 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
2.85
-
L-phenylalanine post-translational activation of PAH in human hepatic cytosol fractions, activator: lysophosphatidylcholine (5 mM), Vmax: 29.1 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
3.01
-
L-phenylalanine post-translational activation of PAH in human hepatic cytosol fractions, activator: H2O2 (0.1 mM), Vmax: 205 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
3.05
-
L-phenylalanine post-translational activation of PAH in human hepatic cytosol fractions, activator: L-Phe (4 mM), Vmax: 14.23 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
3.2
-
S-carboxymethyl-L-cysteine post-translational activation of human cDNA-expressed PAH, activator: HO (hydroxyl radical) (0.01 mM), Vmax: 36.5 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
4.1
-
S-carboxymethyl-L-cysteine post-translational activation of human cDNA-expressed PAH, activator: ONOO- (peroxynitrite)(1 mM), Vmax: 41.27 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
4.38
-
S-carboxymethyl-L-cysteine post-translational activation of PAH in human hepatic cytosol fractions, activator: NO (nitric oxide) (0.01 mM), Vmax: 500 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
4.62
-
S-carboxymethyl-L-cysteine post-translational activation of PAH in human hepatic cytosol fractions, activator: H2O2 (0.1 mM), Vmax: 430 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
5.23
-
S-carboxymethyl-L-cysteine post-translational activation of PAH in human hepatic cytosol fractions, activator: N-ethylmaleimide (50 mM), Vmax: 450 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
6.49
-
S-carboxymethyl-L-cysteine post-translational activation of PAH in human hepatic cytosol fractions, activator: HO (hydroxyl radical) (0.01 mM), Vmax: 455 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
7.54
-
S-carboxymethyl-L-cysteine post-translational activation of PAH in human hepatic cytosol fractions, activator: ONOO- (peroxynitrite)(1 mM), Vmax: 481 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
7.65
-
S-carboxymethyl-L-cysteine post-translational activation of human cDNA-expressed PAH, activator: N-ethylmaleimide (50 mM), Vmax: 5.58 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
8.1
-
S-carboxymethyl-L-cysteine post-translational activation of human cDNA-expressed PAH, activator: L-Phe (4 mM), Vmax: 0.07 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
8.3
-
S-carboxymethyl-L-cysteine post-translational activation of human cDNA-expressed PAH, activator: lysophosphatidylcholine (5 mM), Vmax: 4.38 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
16.53
-
S-carboxymethyl-L-cysteine post-translational activation of PAH in human hepatic cytosol fractions, activator: L-Phe (4 mM), Vmax: 0.87 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
16.53
-
S-carboxymethyl-L-cysteine post-translational activation of PAH in human hepatic cytosol fractions, activator: lysophosphatidylcholine (5 mM), Vmax: 52.31 nmoles/min/mg, pH 6.8, 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
hepatocyte enzyme isolated from human female hepatic cytosolic fractions Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + tetrahydrobiopterin + O2
-
Homo sapiens L-tyrosine + dihydrobiopterin + H2O
-
?
S-carboxymethyl-L-cysteine + tetrahydrobiopterin + O2
-
Homo sapiens S-carboxymethyl-L-cysteine S-oxide + dihydrobiopterin + H2O
-
?

Synonyms

Synonyms Comment Organism
PAH
-
Homo sapiens
phenylalanine hydroxylase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
assay at Homo sapiens