Cloned (Comment) | Organism |
---|---|
gene pah-1, expression as maltose-binding protein fusion protein in Escherichia coli | Caenorhabditis elegans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | gene pah-1 knockout mutant worms do not show an altered phenotype, but in combination with a second mutation of cuticle synthesis, the mutant worms lack a yellow pigment in the cuticle, and show stimulatory effect on superoxide dismutase, and severe cuticle defects, overview | Caenorhabditis elegans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic analysis | Caenorhabditis elegans | |
0.035 | - |
tetrahydrobiopterin | - |
Caenorhabditis elegans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + tetrahydrobiopterin + O2 | Caenorhabditis elegans | mechanism and regulation of the enzyme, which is involved in synthesis of a melamin compound required for cuticle synthesis, overview | L-tyrosine + 4a-hydroxytetrahydrobiopterin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | Q9XYQ5 | wild-type strain N2 and mutant strains, gene pah-1 | - |
Purification (Comment) | Organism |
---|---|
recombinant maltose-binding protein-PAH fusion protein from Escherichia coli, cleavage of the fusion protein by factor Xa, gel filtration | Caenorhabditis elegans |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-phenylalanine + a 5,6,7,8-tetrahydropteridine + O2 = L-tyrosine + a 4a-hydroxy-5,6,7,8-tetrahydropteridine | molecular mechanism | Caenorhabditis elegans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gravid adult | - |
Caenorhabditis elegans | - |
hypodermis | enzyme expression | Caenorhabditis elegans | - |
larva | - |
Caenorhabditis elegans | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + tetrahydrobiopterin + O2 | - |
Caenorhabditis elegans | L-tyrosine + 4a-hydroxytetrahydrobiopterin | - |
? | |
L-phenylalanine + tetrahydrobiopterin + O2 | mechanism and regulation of the enzyme, which is involved in synthesis of a melamin compound required for cuticle synthesis, overview | Caenorhabditis elegans | L-tyrosine + 4a-hydroxytetrahydrobiopterin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PAH | - |
Caenorhabditis elegans |
phenylalanine hydroxylase | - |
Caenorhabditis elegans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Caenorhabditis elegans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Caenorhabditis elegans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
tetrahydrobiopterin | - |
Caenorhabditis elegans |