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Literature summary for 1.14.16.1 extracted from

  • Pavon, J.A.; Fitzpatrick, P.F.
    Insights into the catalytic mechanisms of phenylalanine and tryptophan hydroxylase from kinetic isotope effects on aromatic hydroxylation (2006), Biochemistry, 45, 11030-11037.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.16.1

Cloned(Commentary)

Cloned (Comment) Organism
expression of catalytic core mutant DELTA 117PheH and point mutants thereof in Escherichia coli strain C41(DE3) Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
E280A site-directed mutagenesis of catalytic core mutant DELTA117PheH, 70% reduced activity but unaltered isotopic effects of isotope substrates Rattus norvegicus
F263A site-directed mutagenesis of catalytic core mutant DELTA117PheH, 85% reduced activity but unaltered isotopic effects of isotope substrates Rattus norvegicus
additional information construction and catalytic properties of deletion mutant DELTA117PheH, consisting of the catalytic core of the enzyme, the isotopic effects of substrates [4-2H]-, [3,5-2H2]-, and 2H5-phenylalanine are unaltered compared to the wild-type enzyme, overview Rattus norvegicus
V379D site-directed mutagenesis of catalytic core mutant DELTA117PheH, the isotopic effects of substrates [4-2H]-, [3,5-2H2]-, and 2H5-phenylalanine are altered compared to the wild-type enzyme, overview Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.063
-
 6-methyl-tetrahydrobiopterin pH 7.0, 25°C, recombinant mutant DELTA 117PheH V379D Rattus norvegicus
0.083
-
 6-methyl-tetrahydrobiopterin pH 7.0, 25°C, recombinant mutant DELTA 117PheH Rattus norvegicus
0.12
-
 L-phenylalanine pH 7.0, 25°C, with cofactor 6-methyl-tetrahydrobiopterin, recombinant mutant DELTA 117PheH Rattus norvegicus
0.42
-
 L-phenylalanine pH 7.0, 25°C, with cofactor 6-methyl-tetrahydrobiopterin, recombinant mutant DELTA 117PheH V379D Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-phenylalanine + 5,6,7,8-tetrahydrobiopterin + O2 Rattus norvegicus
-
 L-tyrosine + 4a-hydroxy-tetrahydrobiopterin
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant catalytic core mutant DELTA 117PheH and point mutants thereof from Escherichia coli strain C41(DE3) Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
L-phenylalanine + a 5,6,7,8-tetrahydropteridine + O2 = L-tyrosine + a 4a-hydroxy-5,6,7,8-tetrahydropteridine catalytic mechanism Rattus norvegicus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + 5,6,7,8-tetrahydrobiopterin + O2
-
 Rattus norvegicus L-tyrosine + 4a-hydroxy-tetrahydrobiopterin
-
 ?
L-phenylalanine + 5,6,7,8-tetrahydrobiopterin + O2 specific for the L-isomer, no activity with the D-isomer, effects of isotopic substrates [4-2H]-, [3,5-2H2]-, and 2H5-phenylalanine, overview Rattus norvegicus L-tyrosine + 4a-hydroxy-tetrahydrobiopterin
-
 ?
L-phenylalanine + 6-methyl-tetrahydrobiopterin + O2
-
 Rattus norvegicus L-tyrosine + 6-methyl-4-hydroxy-tetrahydrobiopterin
-
 ?

Synonyms

Synonyms Comment Organism
PheH
-
 Rattus norvegicus
phenylalanine hydroxylase
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.031 0.51 6-methyl-tetrahydrobiopterin pH 7.0, 25°C, recombinant mutant DELTA 117PheH Rattus norvegicus
0.04 1.97 L-phenylalanine pH 7.0, 25°C, with cofactor 6-methyl-tetrahydrobiopterin, recombinant mutant DELTA 117PheH Rattus norvegicus
0.3
-
 L-phenylalanine pH 7.0, 25°C, with cofactor 6-methyl-tetrahydrobiopterin, recombinant mutant DELTA 117PheH V379D Rattus norvegicus
1.12
-
 6-methyl-tetrahydrobiopterin pH 7.0, 25°C, recombinant mutant DELTA 117PheH V379D Rattus norvegicus
7.85
-
 6-methyl-tetrahydrobiopterin pH 7.0, 25°C, recombinant mutant DELTA 117PheH Rattus norvegicus
8.55
-
 L-phenylalanine pH 7.0, 25°C, with cofactor 6-methyl-tetrahydrobiopterin, recombinant mutant DELTA 117PheH Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
5,6,7,8-tetrahydrobiopterin
-
 Rattus norvegicus
6-methyl-tetrahydrobiopterin
-
 Rattus norvegicus